Literature summary for 3.4.22.B37 extracted from

Kovacs, L.; Alexa, A.; Klement, E.; Kokai, E.; Tantos, A.; Gogl, G.; Sperka, T.; Medzihradszky, K.F.; Toezser, J.; Dombradi, V.; Friedrich, P.
Regulation of calpain B from Drosophila melanogaster by phosphorylation (2009), FEBS J., 276, 4959-4972.

Search for more literature for 3.4.22.B37

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
C314A	inactive	Drosophila melanogaster
S845E	the mutation causes a small but reproducible increase in the activation of calpain B	Drosophila melanogaster
T747E	the mutation causes a large increase in the activation of calpain B	Drosophila melanogaster

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	a 6.4 mM free Ca2+ concentration is required for the half maximal activation of calpain B	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	Q9VT65	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation elevates the Ca2+ sensitivity of the protease. The activation of the extracellular signal-regulated protein kinase pathway by extracellular signals results in the phosphorylation and activation of calpain B in fruit flies	Drosophila melanogaster

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Drosophila melanogaster

Source Tissue

Source Tissue	Comment	Organism	Textmining
SCHNEIDER-2 cell	-	Drosophila melanogaster	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
microtubule-associated protein 2c + H2O	-	Drosophila melanogaster	?	-	?
N-succinyl-L-Leu-L-Tyr-7-amido-4-methylcoumarin + H2O	-	Drosophila melanogaster	N-succinyl-L-Leu-L-Tyr + 7-amino-4-methyl-coumarin	-	?

Synonyms

Synonyms	Comment	Organism
calpain B	-	Drosophila melanogaster

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Release 2023.1 (January 2023)