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Literature summary for 3.4.22.B27 extracted from

  • Maemoto, Y.; Kiso, S.; Shibata, H.; Maki, M.
    Analysis of limited proteolytic activity of calpain-7 using non-physiological substrates in mammalian cells (2013), FEBS J., 280, 2594-2607.
    View publication on PubMed
Search for more literature for 3.4.22.B27

Activating Compound

Activating Compound Comment Organism Structure
additional information endosomal sorting complex required for transport (ESCRT) system proteins increase the enzyme activity and autolysis, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GFP-tagged enzyme, recombinant overexpression of wild-type and mutant enzymes in HEK-293T cells, coexpression with FLAG-tagged full-length and C-terminally truncated ALG-2-interacting protein X Homo sapiens

Protein Variants

Protein Variants Comment Organism
C290S site-directed mutagenesis, active site mutant, catalytically inactive mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endosome membrane
-
 Homo sapiens 10008
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y6W3
-
-

Reaction

Reaction Comment Organism Reaction ID
broad endopeptidase specificity the cleavage site of calpain-7 is P4-LLAK-P1 and P1'-KEGITGP-P7' Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
C-terminally truncated ALG-2-interacting protein X + H2O limited proteolysis of C-terminally truncated ALG-2-interacting protein X , but not full-length ALIX. Importance of the CHMP4-ALIX interaction for ALIX truncated mutant cleavage, CHMP4 proteins are part of the ESCRT system Homo sapiens ?
-
 ?
domain 1 of calpastatin-Bro1 domain fuion protein + H2O the calpain-7 cleavage site is located between ELLAK (207-211) and KEGIT(212-216) at the C-terminal border of sub-domain B Homo sapiens ?
-
 ?
additional information calpain-7 performs autolysis, ESCRT proteins enhance the calpain-7 autolysis. Engineered FLAG-tagged casein-Bro1 domain is no substrate Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
calpain-7
-
 Homo sapiens
CAPN7
-
 Homo sapiens

General Information

General Information Comment Organism
additional information importance of not only the N-terminal microtubule-interacting and trafficking domains but also the C-terminal C2 domain-like domains for proteolytic activity of the enzyme Homo sapiens