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Literature summary for 3.4.22.B1 extracted from

  • Than, M.E.; Helm, M.; Simpson, D.J.; Lottspeich, F.; Huber, R.; Gietl, C.
    The 2.0 A crystal structure and substrate specificity of the KDEL-tailed cysteine endopeptidase functioning in programmed cell death of Ricinus communis endosperm (2004), J. Mol. Biol., 336, 1103-1116.
    View publication on PubMed
Search for more literature for 3.4.22.B1

Crystallization (Commentary)

Crystallization (Comment) Organism
at 2.0 A resolution and refined to a R-factor of 18.1%, vapor difussion method Ricinus communis

Inhibitors

Inhibitors Comment Organism Structure
E64
-
 Ricinus communis
H-D-Val-Leu-Lys-chloromethylketone
-
 Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24930
-
 LC-MS experiments, purified enzyme including the inhibitor H-D-Val-Leu-Lys-chloromethylketone Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis O65039
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, gel filtration Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
endosperm ricinosomes Ricinus communis
-
seed
-
 Ricinus communis
-

Storage Stability

Storage Stability Organism
20°C, pH 7.5, 2 hours Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-(Xaa)n-Tyr(NO2)-Asp-OH + H2O
-
 Ricinus communis ?
-
 ?
beta-casein + H2O
-
 Ricinus communis ?
-
 ?
CBZ-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Ricinus communis ?
-
 ?
additional information neutral amino acid residues with large aliphatic and non-polar or aromatic side-chains at the P2 position are good substrates Ricinus communis ?
-
 ?

Synonyms

Synonyms Comment Organism
CysEp
-
 Ricinus communis
cysteine endopeptidase
-
 Ricinus communis