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Literature summary for 3.4.22.71 extracted from
- Elucidation of the recognition sequence of sortase B from Bacillus anthracis by using a newly developed liquid chromatography-mass spectrometry-based method (2017), Biochemistry, 56, 2641-2650 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | method for sequence specificity determination of sortases or other bond-forming enzymes that recognize an amino acid sequence. Using mixtures of recognition peptides of limited complexity, which are reacted with biotinylated substrates, the biotinylated transpeptidation products are isolated with magnetic streptavidin beads and analyzed via liquid chromatography and mass spectrometry | Bacillus anthracis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus anthracis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 2fold stimulation | Bacillus anthracis |  |
| Mg2+ | about 2fold stimulation | Bacillus anthracis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNPNTGDE + H2O | - |
Bacillus anthracis | ? | - |
? | |
| IsdC + H2O | IsdC is a surface protein necessary for heme iron uptake in Bacillus anthracis. SrtB recognizes the NPKTG sequence present | Bacillus anthracis | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Bacillus anthracis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
no residual activity | Bacillus anthracis |
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Release 2023.1 (January 2023)
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