Literature summary for 3.4.22.70 extracted from

Kuropka, B.; Royla, N.; Freund, C.; Krause, E.
Sortase A mediated site-specific immobilization for identification of protein interactions in affinity purification-mass spectrometry experiments (2015), Proteomics, 15, 1230-1234.

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Application

Application	Comment	Organism
additional information	sortase A mediates site-specific immobilization for identification of protein interactions in affinity purification-mass spectrometry experiments, overview. the enzyme is used for proteins or peptide baits with a C-terminal LPXTG recognition motif that are covalently immobilized to beads carrying an oligoglycine peptide (nucleophile). Tryptic on-bead digestion and spike-in of a heavy isotope labeled reference peptide (13C515N1-Val) allows for the absolute quantification of immobilized peptide or protein by MALDI-MS, method overview	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

General Information

General Information	Comment	Organism
additional information	sortase A mediates site-specific immobilization for identification of protein interactions in affinity purification-mass spectrometry experiments, overview	Staphylococcus aureus

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Release 2023.1 (January 2023)