Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
H126A | inactive | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
o-aminobenzoyl-LPETG-2,4-dinitrophenyl | sortase A DELTA64 (residues Asp65Lys210), in 20 mM HEPES, pH 7.5 | Bacillus anthracis | |
0.038 | - |
o-aminobenzoyl-LPETG-2,4-dinitrophenyl | sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 | Bacillus anthracis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon His-affinity resin column chromatography and Sephacryl-100 gel filtration | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SrtA recognizes the LPXTG-sorting signal through a lock-in-key mechanism | Bacillus anthracis | ? | - |
? | |
o-aminobenzoyl-LPETG-2,4-dinitrophenyl + H2O | - |
Bacillus anthracis | o-aminobenzoyl-LPETG + 2,4-dinitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SrtA | - |
Bacillus anthracis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000067 | - |
o-aminobenzoyl-LPETG-2,4-dinitrophenyl | sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 | Bacillus anthracis | |
0.0000083 | - |
o-aminobenzoyl-LPETG-2,4-dinitrophenyl | sortase A DELTA64 (residues Asp65Lys210), in 20 mM HEPES, pH 7.5 | Bacillus anthracis |
General Information | Comment | Organism |
---|---|---|
physiological function | Bacillus anthracis uses the sortase A enzyme to anchor proteins to its cell wall envelope during vegetative growth | Bacillus anthracis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000183 | - |
o-aminobenzoyl-LPETG-2,4-dinitrophenyl | sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 | Bacillus anthracis | |
0.000233 | - |
o-aminobenzoyl-LPETG-2,4-dinitrophenyl | sortase A DELTA64 (residues Asp65Lys210), in 20 mM HEPES, pH 7.5 | Bacillus anthracis |