Cloned (Comment) | Organism |
---|---|
N-terminally His6-tagged SrtADELTAN24 is expressed in Escherichia coli BL21 | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | marked changes in the specificity profile of SrtA are obtained by replacing the beta6/beta7 loop in SrtA with the corresponding domain from SrtB. The chimeric beta6/beta7 loop swap enzyme (SrtLS) conferrs the ability to acylate NPQTN-containing substrates, with a kcat/Kmapp of 0.0062 /M * s. This enzyme is unable to perform the transpeptidation stage of the reaction, suggesting that additional domains are required for transpeptidation to occur. The overall catalytic specificity profile (kcat/Kmapp (NPQTN)/kcat/Kmapp (LPETG)) of SrtLS is altered 700000fold from SrtA. These results indicate that the beta6/beta7 loop is an important site for substrate recognition in sortases | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
N-terminally His6-tagged SrtADELTAN24 expressed in Escherichia coli BL21 | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-KVENPQTNAGT-Dap(Dnp)-NH2 + GGGGG | - |
Staphylococcus aureus | Abz-KVENPQTGGGGG + Gly-Dap(DNP)-NH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SrtA | - |
Staphylococcus aureus |