Literature summary for 3.4.22.70 extracted from

Zhu, J.; Lu, C.; Standland, M.; Lai, E.; Moreno, G.N.; Umeda, A.; Jia, X.; Zhang, Z.
Single mutation on the surface of Staphylococcus aureus sortase A can disrupt its dimerization (2008), Biochemistry, 47, 1667-1674.

Search for more literature for 3.4.22.70

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
I123G	mutant is generated by using wild type truncated protein SrtADELTAN59 as a template. Mutation reduces dimerization	Staphylococcus aureus
K137A	mutant is generated by using wild type truncated protein SrtADELTAN59 as a template. Mutation completely disrupts dimerization	Staphylococcus aureus
K152A	mutant is generated by using wild type truncated protein SrtADELTAN59 as a template. Mutation increases dimerization	Staphylococcus aureus
K62A	mutant is generated by using wild type truncated protein SrtADELTAN59 as a template	Staphylococcus aureus
N132A	mutant is generated by using wild type truncated protein SrtADELTAN59 as a template. Mutation completely disrupts dimerization	Staphylococcus aureus
P126G	mutant is generated by using wild type truncated protein SrtADELTAN59 as a template. Mutation reduces dimerization	Staphylococcus aureus
Y143A	mutant is generated by using wild type truncated protein SrtADELTAN59 as a template. Mutation completely disrupts dimerization	Staphylococcus aureus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
of SrtADELTAN59 and mutant proteins	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Abz-LPETG-Dap(Dnp)-NH2 + GGGGG	-	Staphylococcus aureus	Abz-LPETGGGGGG + Gly-Dap(Dnp)-NH2	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
SrtA	-	Staphylococcus aureus

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Release 2023.1 (January 2023)