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Literature summary for 3.4.22.70 extracted from

  • Frankel, B.A.; Tong, Y.; Bentley, M.L.; Fitzgerald, M.C.; McCafferty, D.G.
    Mutational analysis of active site residues in the Staphylococcus aureus transpeptidase SrtA (2007), Biochemistry, 46, 7269-7278.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and variant SrtA proteins Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
C184S 2700fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
D185A 1.3 fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
D186A 1.8fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
H120A 96000fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
H120Q 36000fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
I182A mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 28fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
I182S 74fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
L181A mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 7.6fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
R197A 540fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
R197K 1000fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
T180A mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 14fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
T183A 1200fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.79
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.8
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
4.7
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
6.7
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
8.5
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
10.6
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide wild-type enzyme Staphylococcus aureus
11.2
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-LPETG-Dap(Dnp)-NH2 + GGGGG
-
Staphylococcus aureus ?
-
?

Synonyms

Synonyms Comment Organism
SrtA
-
Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000019
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
0.0003
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
0.00046
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
0.0006
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
0.69
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide wild-type enzyme Staphylococcus aureus
0.72
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
0.78
-
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus