Cloned (Comment) | Organism |
---|---|
wild-type and variant SrtA proteins | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
C184S | 2700fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
D185A 1.3 | fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
D186A | 1.8fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
H120A | 96000fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
H120Q | 36000fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
I182A | mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 28fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
I182S | 74fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
L181A | mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 7.6fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
R197A | 540fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
R197K | 1000fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
T180A | mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 14fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
T183A | 1200fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.8 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
4.7 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
6.7 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
8.5 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
10.6 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | wild-type enzyme | Staphylococcus aureus | |
11.2 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-LPETG-Dap(Dnp)-NH2 + GGGGG | - |
Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SrtA | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000019 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
0.0003 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
0.00046 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
0.0006 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
0.69 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | wild-type enzyme | Staphylococcus aureus | |
0.72 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
0.78 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus |