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Literature summary for 3.4.22.70 extracted from

  • Gaspar, A.H.; Marraffini, L.A.; Glass, E.M.; Debord, K.L.; Ton-That, H.; Schneewind, O.
    Bacillus anthracis sortase A (SrtA) anchors LPXTG motif-containing surface proteins to the cell wall envelope (2005), J. Bacteriol., 187, 4646-4655.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.70

Application

Application Comment Organism
medicine Bacillus anthracis SrtA anchors surface proteins bearing LPXTG motif sorting signals to the cell wall envelope of vegetative bacilli, srtA gene of Bacillus anthracis is not required for the development of acute anthrax disease in A/J mice Bacillus anthracis

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the plasmid vector pQE30 Bacillus anthracis

Protein Variants

Protein Variants Comment Organism
additional information srtA mutants AHG263 (srtA gene deleted by allelic replacement with ermC marker) and AHG188 Bacillus anthracis

Inhibitors

Inhibitors Comment Organism Structure
(2-(trimethylammonium)ethyl)methanethiosulfonate inhibition at 5 mM, inhibition is relieved by supplementing the reaction with 10 mM dithiothreitol Bacillus anthracis

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 SDS-PAGE, immune-reactive species Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
 parent strain Sterne 34F2
-

Purification (Commentary)

Purification (Comment) Organism
by Ni-NTA affinity chromatography Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-LPATG-diaminopropionic acid + H2O efficient cleavage Bacillus anthracis 2-aminobenzoyl-LPAT + G-diaminopropionic acid
-
 ?
2-aminobenzoyl-LPNTA-diaminopropionic acid + H2O small amount of cleavage Bacillus anthracis 2-aminobenzoyl-LPNT + A-diaminopropionic acid
-
 ?
Abz-LPETG-Dap(Dnp) + H2O cleavage between the threonine and the glycine residues, efficient cleavage Bacillus anthracis Abz-LPET + G-Dap(Dnp)
-
 ?
additional information no cleavage of 2-aminobenzoyl-NPKTG-diaminopropionic acid and 2-aminobenzoyl-LGATG-diaminopropionic acid Bacillus anthracis ?
-
 ?

Synonyms

Synonyms Comment Organism
SrtA
-
 Bacillus anthracis