Protein Variants | Comment | Organism |
---|---|---|
N98A | kcat/Km is 1.2fold lower than wild-type value | Staphylococcus aureus |
N98Q | kcat/Km is 1.1fold higher than wild-type value | Staphylococcus aureus |
R197A | kcat/Km is 2769fold lower than wild-type value | Staphylococcus aureus |
R197H | kcat/Km is 610fold lower than wild-type value | Staphylococcus aureus |
R197K | kcat/Km is 2571fold lower than wild-type value | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00824 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, wild-type enzyme | Staphylococcus aureus | |
0.00932 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme N98Q | Staphylococcus aureus | |
0.0097 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme N98A | Staphylococcus aureus | |
0.01391 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme R197K | Staphylococcus aureus | |
0.01597 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme R197A | Staphylococcus aureus | |
0.0168 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme R197H | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-LPETG-Dap(Dnp) + H2O | - |
Staphylococcus aureus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00019 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme R197K | Staphylococcus aureus | |
0.00021 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme R197A | Staphylococcus aureus | |
0.00098 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme R197H | Staphylococcus aureus | |
0.29 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme N98A | Staphylococcus aureus | |
0.3 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, wild-type enzyme | Staphylococcus aureus | |
0.37 | - |
Abz-LPETG-Dap(Dnp) | 37°C, pH 7.5, mutant enzyme N98Q | Staphylococcus aureus |