Cloned (Comment) | Organism |
---|---|
wild-type and mutant glutathione S-transferase-fusion constructs are transformed into Escherichia coli BL21 cell strain for overexpression | Severe acute respiratory syndrome-related coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
C300A | mutant enzyme shows more than 30% of wild-type activity | Severe acute respiratory syndrome-related coronavirus |
E288A | mutant enzyme retains less than 10% of the wild-type activity | Severe acute respiratory syndrome-related coronavirus |
F291A | activity is higher than that of wild-type enzyme | Severe acute respiratory syndrome-related coronavirus |
G283A | no significant activity differences from the wild-type protease | Severe acute respiratory syndrome-related coronavirus |
I286A | activity is higher than that of wild-type enzyme | Severe acute respiratory syndrome-related coronavirus |
L282A | mutant enzyme shows more than 30% of wild-type activity | Severe acute respiratory syndrome-related coronavirus |
N214A | mutant enzyme shows more than 30% of wild-type activity | Severe acute respiratory syndrome-related coronavirus |
N289A | mutant enzyme retains less than 10% of the wild-type activity | Severe acute respiratory syndrome-related coronavirus |
Q290A | mutant enzyme retains less than 10% of the wild-type activity | Severe acute respiratory syndrome-related coronavirus |
Q299A | mutant enzyme retains less than 10% of the wild-type activity | Severe acute respiratory syndrome-related coronavirus |
R298A | mutant enzyme retains less than 10% of the wild-type activity | Severe acute respiratory syndrome-related coronavirus |
S284A | activity is higher than that of wild-type enzyme | Severe acute respiratory syndrome-related coronavirus |
S284A/T285A/I286A | activity is 3.7fold higher than wild-type activity | Severe acute respiratory syndrome-related coronavirus |
S301A | no significant activity differences from the wild-type protease | Severe acute respiratory syndrome-related coronavirus |
T280A | no significant activity differences from the wild-type protease | Severe acute respiratory syndrome-related coronavirus |
T285A | activity is higher than that of wild-type enzyme | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGF RKME-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] + H2O | - |
Severe acute respiratory syndrome-related coronavirus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SARS 3C-like protease | - |
Severe acute respiratory syndrome-related coronavirus |