Literature summary for 3.4.22.68 extracted from

Malakhov, M.P.; Mattern, M.R.; Malakhova, O.A.; Drinker, M.; Weeks, S.D.; Butt, T.R.
SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins (2004), J. Struct. Funct. Genomics, 5, 75-86.

Search for more literature for 3.4.22.68

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Gu-HCl	500 mM reduces cleavage to 60%, 1 M reduces cleavage to 0%	Saccharomyces cerevisiae
additional information	no inhibitory effects are observed with Triton X100 (1 M), imidazole (300 mM), reduced glutathione (20 mM), maltose (20 mM), glycerol (20% v/v), ethylene glycol (20% v/v), sucrose (20% w/v), ethanol (10% v/v)	Saccharomyces cerevisiae
NaCl	500 mM reduces cleavage to 60%, 1 M reduces cleavage to 30%	Saccharomyces cerevisiae
Urea	2 M reduces cleavage to 95%, 3 M reduces cleavage to 5%	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	x * 31000, SDS-PAGE	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q02724	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Storage Stability

Storage Stability	Organism
-70°C, 50% glycerol, 75 mM Tris-HCl pH 8.0, 0.5 mM DTT, 1 mM EDTA, 6 months, no detectable decay of activity	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
small ubiquitin-like modifier protein + H2O	-	Saccharomyces cerevisiae	?	Ulp1 catalyzes the proteolytic processing of SUMO to its mature form	?
SUMO-GFP fusion substrate + H2O	pH 8.0, 25°C, in the presence of 5 mM 2-mercaptoethanol	Saccharomyces cerevisiae	SUMO + GFP	-	?
SUMO-MMP13 + H2O	cleavage occurs only to 60%	Saccharomyces cerevisiae	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 31000, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
SUMO protease 1	-	Saccharomyces cerevisiae
Ulp1	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	37	-	Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
4	37	-	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	9	-	Saccharomyces cerevisiae

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)