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Literature summary for 3.4.22.65 extracted from

  • Al-Ghouleh, A.; Johal, R.; Sharquie, I.; Emara, M.; Harrington, H.; Shakib, F.; Ghaemmaghami, A.
    The glycosylation pattern of common allergens: The recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent (2012), PLoS ONE, 7, e33929.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Pichia pastoris Dermatophagoides pteronyssinus

Organism

Organism UniProt Comment Textmining
Dermatophagoides pteronyssinus P08176
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-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein analysis of the glycosylation pattern and comparison to other allergens, mannosylation appears to be a dominant feature among allergens, overview. The main dominant sugars on these allergens are 1-2, 1-3 and 1-6 mannose, the sugar part of the enzyme is recognized by lectins from Datura stramonium lectin, Arachis hypogaea, and Sambucus nigra. Sodium periodate deglycosylation of natural and recombinant enzyme Der p 1 resulting in a significant decrease in the uptake of both Der p 1 preparations into dendritic cells. The recombinant enzyme shows a higher degree of mannans compared to the native enzyme Dermatophagoides pteronyssinus

Synonyms

Synonyms Comment Organism
Der p 1
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 Dermatophagoides pteronyssinus

General Information

General Information Comment Organism
evolution mannosylation is a unique feature of the allergens within the cysteine protease family of enzymes, e.g. Der 1 p, papain, and bromelain, while non-allergens of the family, e.g. calpain, and cysteine protease B do not show mannosylation in their carbohydrate patterns, staphopain B is also a cysteine protease protein, like Der p 1, but is an amannosylated antigen Dermatophagoides pteronyssinus
additional information the enzyme is bound by the mannose receptor of dendritic cells, the recombinant enzyme binds stronger due to its higher degree of mannans compared to the native enzyme Dermatophagoides pteronyssinus
physiological function stimulation with natural Der p 1 causes an increase in thymic stromal lymphopoietin, TSLP, cytokine secretion by human lung epithelia in a carbohydrate-dependent manner. TSLP may drive denditic cell maturation in support of allergic hypersensitivity reactions Dermatophagoides pteronyssinus