Cloned (Comment) | Organism |
---|---|
recombinant expression in Pichia pastoris | Dermatophagoides pteronyssinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dermatophagoides pteronyssinus | P08176 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | analysis of the glycosylation pattern and comparison to other allergens, mannosylation appears to be a dominant feature among allergens, overview. The main dominant sugars on these allergens are 1-2, 1-3 and 1-6 mannose, the sugar part of the enzyme is recognized by lectins from Datura stramonium lectin, Arachis hypogaea, and Sambucus nigra. Sodium periodate deglycosylation of natural and recombinant enzyme Der p 1 resulting in a significant decrease in the uptake of both Der p 1 preparations into dendritic cells. The recombinant enzyme shows a higher degree of mannans compared to the native enzyme | Dermatophagoides pteronyssinus |
Synonyms | Comment | Organism |
---|---|---|
Der p 1 | - |
Dermatophagoides pteronyssinus |
General Information | Comment | Organism |
---|---|---|
evolution | mannosylation is a unique feature of the allergens within the cysteine protease family of enzymes, e.g. Der 1 p, papain, and bromelain, while non-allergens of the family, e.g. calpain, and cysteine protease B do not show mannosylation in their carbohydrate patterns, staphopain B is also a cysteine protease protein, like Der p 1, but is an amannosylated antigen | Dermatophagoides pteronyssinus |
additional information | the enzyme is bound by the mannose receptor of dendritic cells, the recombinant enzyme binds stronger due to its higher degree of mannans compared to the native enzyme | Dermatophagoides pteronyssinus |
physiological function | stimulation with natural Der p 1 causes an increase in thymic stromal lymphopoietin, TSLP, cytokine secretion by human lung epithelia in a carbohydrate-dependent manner. TSLP may drive denditic cell maturation in support of allergic hypersensitivity reactions | Dermatophagoides pteronyssinus |