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Literature summary for 3.4.22.65 extracted from

  • Mueller, G.A.; Edwards, L.L.; Aloor, J.J.; Fessler, M.B.; Glesner, J.; Pomes, A.; Chapman, M.D.; London, R.E.; Pedersen, L.C.
    The structure of the dust mite allergen Der p 7 reveals similarities to innate immune proteins (2010), J. Allergy Clin. Immunol., 125, 909-917.e4.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of Der p 7-MBP fusion protein and His6-tagged Der p 7 in Escherichia coli Rosetta2(DE3) pLacI cells Dermatophagoides pteronyssinus

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant Der p 7-MBP fusion protein, mixing of 0.001 ml of 27 mg/mL protein in 25 mM HEPES, pH 7.4, 75 mM NaCl, and 5 mM maltose, with 00.001 ml of reservoir solution containing 42.5 mM Tris pH 8.5, 12.75% PEG 4000, 85 mM lithium sulfate, and 7.5% glycerol, 4°C, 1 week, X-ray diffraction structure determination and analysis at 2.35 A resolution, molecular replacement Dermatophagoides pteronyssinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22200
-
 x * 22200, about, mature Der p 7, sequence calculation, x * 24000-31000, SDS-PAGE Dermatophagoides pteronyssinus

Organism

Organism UniProt Comment Textmining
Dermatophagoides pteronyssinus P49273
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein different levels of glycosylation or isozymes Dermatophagoides pteronyssinus

Purification (Commentary)

Purification (Comment) Organism
recombinant Der p 7-MBP fusion protein Der p 7 from Escherichia coli Rosetta2(DE3) pLacI cells by amylose affinity chromatography and gel filtration. Recombinant His6-tagged Der p 7 from Escherichia coli Rosetta2(DE3) pLacI cells by alternating nickel affinity and anion exchange chromatography Dermatophagoides pteronyssinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Der p 7 binds a bacterially-derived lipid product, a common feature of some allergens, ligand binding analysis by NMR analysis Dermatophagoides pteronyssinus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 22200, about, mature Der p 7, sequence calculation, x * 24000-31000, SDS-PAGE Dermatophagoides pteronyssinus
More Der p 7 has an elongated structure with two 4-stranded anti-parallel beta-sheets which wrap around a long C-terminal helix, NMR analysis of His-tagged Der p 7, overview Dermatophagoides pteronyssinus

Synonyms

Synonyms Comment Organism
Der p 7
-
 Dermatophagoides pteronyssinus
dust mite allergen Der p 7
-
 Dermatophagoides pteronyssinus

General Information

General Information Comment Organism
additional information the Group 7 as well as the Group 2 mite allergens are structurally similar to different proteins in the TLR pathway Dermatophagoides pteronyssinus
physiological function Der p 7 elicits strong IgE antibody and T-cell responses in mite allergic patients. Sensitization to house dust mite allergens is strongly correlated with asthma. The allergen function may contribute to allergenicity, e.g. the protease activity of Group 1 mite allergens, and the interaction with the innate immune system by Group 2 mite allergens Dermatophagoides pteronyssinus