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Literature summary for 3.4.22.65 extracted from

  • Takai, T.; Kato, T.; Ota, M.; Yasueda, H.; Kuhara, T.; Okumura, K.; Ogawa, H.
    Recombinant Der p 1 and Der f 1 with in vitro enzymatic activity to cleave human CD23, CD25 and alpha1-antitrypsin, and in vivo IgE-eliciting activity in mice (2005), Int. Arch. Allergy Immunol., 137, 194-200.
    View publication on PubMed
Search for more literature for 3.4.22.65

Application

Application Comment Organism
medicine proteolytic activity in pathogenesis of allergens Dermatophagoides pteronyssinus
medicine Der f 1 is one of the most potent indoor allergens from house dust mites, shows IgE-eliciting activity in mice, recombinant DER f 1-N53Q will be a useful tool as alternatives to the natural Der f1 for in vitro and in vivo analyses Dermatophagoides farinae

Cloned(Commentary)

Cloned (Comment) Organism
recombinant proforms secreted into culture supernatant of Pichia pastoris cells and converted into prosequence-removed mature form Der p 1-WT Dermatophagoides pteronyssinus

Protein Variants

Protein Variants Comment Organism
additional information mutant Der f 1-N53Q, in which the N-glycosylation motif within the mature sequence is disrupted Dermatophagoides farinae
N52Q mutant with disrupted N-glycosylation motif in the prodomain, lower productivity Dermatophagoides pteronyssinus

Inhibitors

Inhibitors Comment Organism Structure
E-64 cysteine protease specific inhibitor Dermatophagoides farinae
E-64 cysteine protease-specific inhibitor, complete inhibition Dermatophagoides pteronyssinus

Organism

Organism UniProt Comment Textmining
Dermatophagoides farinae
-
-
-
Dermatophagoides pteronyssinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dermatophagoides pteronyssinus
mature form purified by anion exchange and gel filtration chromatography Dermatophagoides farinae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha1-antitrypsin + H2O recombinant Der p 1 previously activated with L-cysteine or DTT Dermatophagoides pteronyssinus ?
-
 ?
Azocoll + H2O
-
 Dermatophagoides pteronyssinus ?
-
 ?
Azocoll + H2O collagen substrate, Der f 1-N53Q proteolytically active Dermatophagoides farinae ?
-
 ?
butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O short synthetic substrate Dermatophagoides farinae butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
CD23 + H2O low-affinity IgE receptor Dermatophagoides pteronyssinus ?
-
 ?
CD25 + H2O alpha-subunit of the IL-2 receptor Dermatophagoides pteronyssinus ?
-
 ?
human alpha1-antitrypsin + H2O cleaved by Der f 1-N53Q but not without its activation Dermatophagoides farinae ?
-
 ?
human CD23 + H2O cleaved by Der f 1-N53Q and natural Der f 1 on the cell surface in a time- and dose dependent manner Dermatophagoides farinae ?
-
 ?
human CD25 + H2O cleaved by Der f 1-N53Q and natural Der f 1 on the cell surface in a time- and dose dependent manner Dermatophagoides farinae ?
-
 ?
N-tert-butoxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Dermatophagoides pteronyssinus N-tert-butoxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
Der f 1
-
 Dermatophagoides farinae
Der p 1
-
 Dermatophagoides pteronyssinus
major house dust mite allergen
-
 Dermatophagoides pteronyssinus