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Literature summary for 3.4.22.62 extracted from

  • Yamakawa, N.; Takahashi, A.; Mori, E.; Imai, Y.; Furusawa, Y.; Ohnishi, K.; Kirita, T.; Ohnishi, T.
    High LET radiation enhances apoptosis in mutated p53 cancer cells through Caspase-9 activation (2008), Cancer Sci., 99, 1455-1460.
    View publication on PubMed
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Application

Application Comment Organism
pharmacology studies on mechanisms of apoptosis induced by high linear energy transfer (LET) radiation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
benzyloxycarbonyl-LEHD-fluoromethylketone i.e. z-LEHD-FMK, inhibitor effect on apoptosis induced by high linear energy transfer (LET) radiation, induction of apoptosis investigated at 48 h after 10 day irradiation in the presence of 2-100 microM inhibitor concentration, caspase-9 inhibitor suppresses caspase-3 activation and apoptosis induction by radiation to a greater extent than caspase-8 inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-caspase-3 + H2O Homo sapiens high linear energy transfer (LET) radiation enhances apoptosis by activation of caspase-3 through caspase-9 caspase-3 + ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
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Source Tissue

Source Tissue Comment Organism Textmining
gingiva gingivial cancer cells (Ca9-22 cells) containing a mutated p53 (mp53) protein by point mutation at codon 248 Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 mechanisms of apoptosis induced by high linear energy transfer (LET) radiation, irradiation with X-rays, C-ion, or Fe-ion beams, apoptosis quantified with Hoechst 33342 staining, activation of caspase-3 analyzed by Western blot and flow cytometry, poly (ADP-ribose) polymerase (PARP) cleavage, caspase-9 inhibitor suppresses caspase-3 activation and induction of apoptosis, high linear energy transfer (LET) radiation enhances apoptosis by activation of caspase-3 through caspase-9, even in the presence of mp53 protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pro-caspase-3 + H2O high linear energy transfer (LET) radiation enhances apoptosis by activation of caspase-3 through caspase-9 Homo sapiens caspase-3 + ?
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 ?