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Literature summary for 3.4.22.62 extracted from

  • Day, T.W.; Huang, S.; Safa, A.R.
    c-FLIP knockdown induces ligand-independent DR5-, FADD-, caspase-8-, and caspase-9-dependent apoptosis in breast cancer cells (2008), Biochem. Pharmacol., 76, 1694-1704.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.62

Application

Application Comment Organism
pharmacology the cellular-FLICE inhibitory protein (c-FLIP) analyzed as a potential therapeutic target for breast cancers Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
benzyloxycarbonyl-LEHD-fluoromethylketone cell transfection with siRNA against the cellular-FLICE inhibitory protein (c-FLIP) followed by the addition of the caspase-9 inhibitor benzyloxycarbonyl-Leu-Glu-His-Asp-fluoromethylketone protects the cells from spontaneous apoptosis, indicates that c-FLIP siRNA-triggered apoptosis occurrs by a caspase-9-dependent process, inhibitor concentration of 20 microM for 48 h, apoptosis determined by counting fragmented nuclei using fluorescent dyes, caspase-9 activation shown by Western blot Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens knockdown of the cellular-FLICE inhibitory protein (c-FLIP) using small interfering RNA (siRNA) triggers ligand-independent caspase-9-dependent spontaneous apoptosis ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
-
-

Source Tissue

Source Tissue Comment Organism Textmining
MCF-7 cell
-
 Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 knockdown of the cellular-FLICE inhibitory protein (c-FLIP) using small interfering RNA (siRNA) triggers ligand-independent caspase-9-dependent spontaneous apoptosis, proliferation of MCF-7 breast cancer cells decreased Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information knockdown of the cellular-FLICE inhibitory protein (c-FLIP) using small interfering RNA (siRNA) triggers ligand-independent caspase-9-dependent spontaneous apoptosis Homo sapiens ?
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 ?
additional information ligand-independent caspase-9-dependent spontaneous apoptosis triggered by knockdown of the cellular-FLICE inhibitory protein (c-FLIP) using small interfering RNA (siRNA) Homo sapiens ?
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 ?