Protein Variants | Comment | Organism |
---|---|---|
DELTA1-111 | a truncated form of procaspase-9 missing the first 111 amino acids, and a variety of mutants derived therefrom are expressed in Echerichia coli inclusion bodies. Upon refolding to active enzyme, DELTA1-111 procaspase-9 and mutants are recovered at purity greater than 95% with a final yield of 20-35 mg/L cell culture. the active procaspaseretains its prosegment, while undergoing major auto processing at ASp315 and a minor cleavage at Glu306 | Homo sapiens |
DELTA1-111/DELTAA316-D330 | activity with acetyl-LEHD-7-amido-4-trifluoromethylcoumarin is 6fold higher than the activity of mutant DELTA1-111 | Homo sapiens |
DELTA1-111/DELTAA316-D330 | activity with acetyl-LEHD-7-amido-4-trifluoromethyl coumarin is 6fold higher than the activity of mutant DELTA1-111 | Homo sapiens |
DELTA1-111/E306A/D315A | E306A/D315A mutation blocks autoprocessing and shifts pH optimum from pH 6.2 for DELTA1-111 to pH 7.2. Activity with acetyl-LEHD-7-amido-4-trifluoromethyl coumarin is identical to the activity of mutant DELTA1-111 | Homo sapiens |
DELTA1-111/E306D/D315A | activity with acetyl-LEHD-7-amido-4-trifluoromethylcoumarin is 90% of the activity of mutant DELTA1-111 | Homo sapiens |
DELTA1-111/E306D/D315A | activity with acetyl-LEHD-7-amido-4-trifluoromethyl coumarin is 90% of the activity of mutant DELTA1-111 | Homo sapiens |
DELTA1-111/E306D/DELTAA316-D330 | activity with acetyl-LEHD-7-amido-4-trifluoromethylcoumarin is 7.6fold higher than the activity of mutant DELTA1-111 | Homo sapiens |
DELTA1-111/E306D/DELTAA316-D330 | activity with acetyl-LEHD-7-amido-4-trifluoromethyl coumarin is 7.6fold higher than the activity of mutant DELTA1-111 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyloxycarbonyl-VAD-fluoromethylketone | - |
Homo sapiens | |
benzyloxycarbonyl-VAE-fluoromethylketone | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-LEHD-7-amido-4-trifluoromethyl coumarin + H2O | - |
Homo sapiens | acetyl-LEHD + 7-amino-4-trifluoromethyl coumarin | - |
? | |
acetyl-LEHD-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | acetyl-LEHD + 7-amino-4-trifluoromethylcoumarin | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
mutant DELTA1-111 | Homo sapiens |
7.2 | - |
mutant DELTA1-111/E306A/D315A | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0142 | - |
benzyloxycarbonyl-VAE-fluoromethylketone | - |
Homo sapiens | |
0.0171 | - |
benzyloxycarbonyl-VAD-fluoromethylketone | - |
Homo sapiens |