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Literature summary for 3.4.22.62 extracted from

  • Yin, Q.; Park, H.H.; Chung, J.Y.; Lin, S.C.; Lo, Y.C.; da Graca, L.S.; Jiang, X.; Wu, H.
    Caspase-9 holoenzyme is a specific and optimal procaspase-3 processing machine (2006), Mol. Cell, 22, 259-268.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information engineering of a leucine-zipper-linked dimeric caspase-9 (LZ-C9). Removal of the caspase recruitment domain of procaspase-9 and replacement with the leucine-zipper dimerization domain of the transcriptional factor GCN4. A six residue linker is added in between the leucine zipper and the beginning of the catalytic domain of caspase-9. LZ-C9 is more active than the caspase-9 holoenzyme for acetyl-LEHD-7-amido-4-trifluoromethylcoumarin but much less active the the caspase-9 holoenzyme for the physiological substrate procaspase-3 Homo sapiens
additional information engineering of a leucine-zipper-linked dimeric caspase-9 (LZ-C9). Removal of the caspase recruitment domain of procaspase-9 and replacement with the leucine-zipper dimerization domain of the transcriptional factor GCN4. A six residue linker is added in between the leucine zipper and the beginning of the catalytic domain of caspase-9. LZ-C9 is more active than the caspase-9 holoenzyme for acetyl-LEHD-7-amido-4-trifluoromethyl coumarin but much less active the the caspase-9 holoenzyme for the physiological substrate procaspase-3 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.139
-
procaspase-3 caspase-9 holoenzyme Homo sapiens
0.466
-
acetyl-LEHD-7-amido-4-trifluoromethylcoumarin leucine-zipper-linked dimeric caspase-9 Homo sapiens
0.466
-
acetyl-LEHD-7-amido-4-trifluoromethyl coumarin leucine-zipper-linked dimeric caspase-9 Homo sapiens
0.686
-
acetyl-LEHD-7-amido-4-trifluoromethylcoumarin caspase-9 holoenzyme Homo sapiens
0.686
-
acetyl-LEHD-7-amido-4-trifluoromethyl coumarin caspase-9 holoenzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procaspase-3 + H2O Homo sapiens
-
caspase-3 + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-LEHD-7-amido-4-trifluoromethyl coumarin + H2O
-
Homo sapiens acetyl-LEHD + 7-amino-4-trifluoromethyl coumarin
-
?
acetyl-LEHD-7-amido-4-trifluoromethylcoumarin + H2O
-
Homo sapiens acetyl-LEHD + 7-amino-4-trifluoromethylcoumarin
-
?
procaspase-3 + H2O
-
Homo sapiens caspase-3 + ?
-
?