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Literature summary for 3.4.22.6 extracted from

  • Baines, B.S.; Brocklehurst, K.; Carey, P.R.; Jarvis, M.; Salih, E.; Storer, A.C.
    Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives (1986), Biochem. J., 233, 119-129.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
 benzyloxycarbonyl-Lys phenyl ester at pH 6.0 Carica papaya
0.01
-
 benzyloxycarbonyl-Lys phenyl ester at pH 6.9 and at pH 4.9 Carica papaya
5
-
 benzoyl-Arg-4-nitroanilide pH 7.4 Carica papaya
8.6
-
 benzoyl-Arg-4-nitroanilide pH 4.0 Carica papaya
13
-
 benzoyl-Arg-4-nitroanilide pH 9.2 Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Carica papaya
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Lys phenyl ester + H2O
-
 Carica papaya benzyloxycarbonyl + phenol
-
 ?
additional information the abnormal catalytic site environment of chymopapain A may have consequences for the acylation step of the catalytic act, does not pertub the conformation of the bound acyl group at the acyl-enzyme-intermediate stage of catalysis Carica papaya ?
-
 ?
N-benzoyl-Arg-4-nitroanilide + H2O
-
 Carica papaya N-benzoyl-Arg + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 benzoyl-Arg-4-nitroanilide pH 4.0 and pH 7.4 Carica papaya
0.05
-
 benzoyl-Arg-4-nitroanilide pH 9.2 Carica papaya
14
-
 benzyloxycarbonyl-Lys phenyl ester at pH 4.9 Carica papaya
15.5
-
 benzyloxycarbonyl-Lys phenyl ester at pH 6.0 and at pH 6.9 Carica papaya