Protein Variants | Comment | Organism |
---|---|---|
D129A | site-directed mutagenesis, inactive mutant | Homo sapiens |
E637A | site-directed mutagenesis, inactive mutant | Homo sapiens |
additional information | generation of stable caspase-6 knockdown cell lines using K562 cells. Mutations at residues D442, D444, D516, D768, and D936 do not prevent cleavage in vitro | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Z-VAD-fluoromethylketone | a Pan caspase inhibitor | Homo sapiens | |
Z-VEID-fluoromethylketone | a caspase-6 inhibitor | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
human telomerase + H2O | Homo sapiens | caspase-6 cleaves human telomerase, hTERT, during apoptosis in cultured cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
JURKAT cell | - |
Homo sapiens | - |
K-562 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
human telomerase + H2O | caspase-6 cleaves human telomerase, hTERT, during apoptosis in cultured cells | Homo sapiens | ? | - |
? | |
human telomerase + H2O | caspase-6 cleaves human telomerase, hTERT, at residues D129 and D637, generation of three major proteolytic fragments, cleavage site determination using caspase-6 resistant substrate mutants D129A, D637A, and D129A/D637A, overview | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the caspase-cleavage motifs in teleomerases from old world monkeys and apes, and the caspase-6 sites appear conserved in all primates | Homo sapiens |
physiological function | telomerase can inhibit or activate apoptosis depending upon cell type and experimental conditions, mechanism for telomerase downregulation through caspase-mediated cleavage | Homo sapiens |