Literature summary for 3.4.22.57 extracted from

Fassy, F.; Krebs, O.; Rey, H.; Komara, B.; Gillard, C.; Capdevila, C.; Yea, C.; Faucheu, C.; Blanchet, A.M.; Miossec, C.; Diu-Hercend, A.
Enzymic activity of two caspases related to interleukin-1beta-converting enzyme (1998), Eur. J. Biochem., 253, 76-83.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in baculovirus-infected insect cells as 30000 Da proteins lacking the propeptide. Automaturation into p20 and p10 subunits occurs within the cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
acetyl-DEVD-aldehyde	-	Homo sapiens
acetyl-YVAD-aldehyde	-	Homo sapiens
cowpox serpin CrmA	-	Homo sapiens
N-tert-butyloxycarbonyl-AEVD-aldehyde	-	Homo sapiens
N-tert-butyloxycarbonyl-IETD-aldehyde	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	succinyl-YVAD-4-nitroanilide	pH 7.5, 37°C	Homo sapiens
0.09	-	succinyl-YVAD-7-amido-4-methylcoumarin	pH 7.5, 37°C	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the production of the enzyme induces activation of CPP32 like caspase	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	expression in baculovirus-infected insect cells as 30000 Da proteins lacking the propeptide. Automaturation into p20 and p10 subunits occurs within the cells	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant T7-tagged enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the production of the enzyme induces activation of CPP32 like caspase	Homo sapiens	?	-	?
pro-interleukin-1beta + H2O	less efficient cleavage than with EC 3.4.22.36, caspase-1. The enzyme cleaves between YVHD116 and A117 to generate the 17500 Da mature active interleukin-1beta. An additional cleavage site between FEAD27 and G28 generates a 28000 Da intermediate form	Homo sapiens	additional information	-	?
succinyl-YVAD-4-nitroanilide + H2O	-	Homo sapiens	succinyl-YVAD + 4-nitroaniline	-	?
succinyl-YVAD-amido-4-methylcoumarin + H2O	-	Homo sapiens	succinyl-YVAD + amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
More	expression in baculovirus-infected insect cells as 30000 Da proteins lacking the propeptide. Automaturation into p20 and p10 subunits occurs within the cells	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.4	-	succinyl-YVAD-amido-4-methylcoumarin	pH 7.5, 37°C	Homo sapiens
1.07	-	succinyl-YVAD-4-nitroanilide	pH 7.5, 37°C	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00002	-	acetyl-YVAD-aldehyde	pH 7.5, 37°C	Homo sapiens
0.00015	-	acetyl-DEVD-aldehyde	pH 7.5, 37°C	Homo sapiens

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Release 2023.1 (January 2023)