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Literature summary for 3.4.22.54 extracted from

  • Ono, Y.; Ojima, K.; Torii, F.; Takaya, E.; Doi, N.; Nakagawa, K.; Hata, S.; Abe, K.; Sorimachi, H.
    Skeletal muscle-specific calpain is an intracellular Na+-dependent protease (2010), J. Biol. Chem., 285, 22986-22998.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.54

Protein Variants

Protein Variants Comment Organism
C129S protease-inactive mutant form Rattus norvegicus
N358D activity-attenuated mutant, in contrast to wild type, which autolyses quickly in the absence of Ca2+, the mutant N358D expressed in Sf-9 cells can be partially purified, N358D is very unstable in solutions with high salt concentrations, autolysis of N358D is NaCl-dependent Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ calpain 3/p94 is activated by Ca2+ and undergoes very rapid autolytic degradation even in the absence of Ca2+ Rattus norvegicus
Na+ calpain 3/p94 is activated by Na+ and undergoes Na+-dependent, but not Cs+-dependent, autolysis in the absence of Ca2+. Na+ and Ca2+ complementarily activate autolysis of calpain3/p94 at physiological concentrations. Na+ and Ca2+ direct calpain3/p94 to proteolyze different substrates Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-actinin-3 + H2O Rattus norvegicus
-
 ?
-
 ?
alpha-fodrin + H2O Rattus norvegicus
-
 ?
-
 ?
tropomyosin + H2O Rattus norvegicus
-
 ?
-
 ?
troponin I + H2O Rattus norvegicus
-
 ?
-
 ?
troponins T + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-actinin-3 + H2O
-
 Rattus norvegicus ?
-
 ?
alpha-fodrin + H2O
-
 Rattus norvegicus ?
-
 ?
tropomyosin + H2O
-
 Rattus norvegicus ?
-
 ?
troponin I + H2O
-
 Rattus norvegicus ?
-
 ?
troponins T + H2O
-
 Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
calpain 3
-
 Rattus norvegicus
p94
-
 Rattus norvegicus