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Literature summary for 3.4.22.54 extracted from

  • Garcia Diaz, B.E.; Gauthier, S.; Davies, P.L.
    Ca2+ dependency of calpain 3 (p84) activation (2006), Biochemistry, 45, 3714-3722.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ P94 insertion sequence 1 is a propeptide that must be autoproteolytically cleaved to provide access of substrates and inhibitors to the enzyme's active site. Initial autoproteolytic cleavage is an intramolecular reaction, transient binding of two Ca2+ ions to the core would be sufficient to promote the reaction that is facilitated by having the scissile peptide close to the active site cysteine. The second autolytic cleavage is much slower and requires higher Ca2+ levels, consistent with it being an intermolecular reaction. High increase in hydrolysis rate when Ca2+ is increased from 10 to 100 mM Homo sapiens
K+ 1 mM, moderate increase of hydrolysis rate Homo sapiens
Mg2+ 1 mM, moderate increase of hydrolysis rate Homo sapiens
Na+ 1 mM, moderate increase of hydrolysis rate Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification P94 insertion sequence 1 is a propeptide that must be autoproteolytically cleaved to provide access of substrates and inhibitors to the enzyme's active site Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-leucine-tyrosine-aminomethylcoumarin + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
calpain 3/p94
-
Homo sapiens
p94
-
Homo sapiens