Activating Compound | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | activates at low concentrations but inhibits above 2 M | Trypanosoma cruzi | |
KSCN | activates at low concentrations but inhibits above 1 M | Trypanosoma cruzi | |
Urea | maximal at 2 M with natural substrates, no activation with synthetic substrates | Trypanosoma cruzi |
General Stability | Organism |
---|---|
unstable in the absence of substrate at pH 8.7 and 37°C | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | activates at low concentrations but inhibits above 2 M | Trypanosoma cruzi | |
KSCN | activates at low concentrations but inhibits above 1 M | Trypanosoma cruzi | |
L-trans-epoxysuccinylleucylamido(4-guanidino)butane | strong inhibitor | Trypanosoma cruzi | |
Urea | 70% inhibition at 5 M | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide | - |
Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | 60000 | SDS-PAGE, depending on electrophoretic conditions | Trypanosoma cruzi |
36300 | - |
calculated from nucleotide sequence | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q598P5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | 1.68 | 4-nitroanilide derivatives | Trypanosoma cruzi |
0.1 | 3.77 | beta-naphthylamide derivatives | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
benzoyl-PFR-4-nitroanilide + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
additional information | substrates are chromogenic peptides with a carboxyl Arg or Lys, enzyme requires at least one more amino acid, preferably Arg, Phe, Val or Leu between the terminal Arg or Lys and the amino-blocking group | Trypanosoma cruzi | ? | - |
? | |
N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide + H2O | - |
Trypanosoma cruzi | N-carbobenzoxy-Arg-Arg + 2-amino-4-methoxynaphthalene | - |
? | |
peptidyl-4-nitroanilide + H2O | derivatives of | Trypanosoma cruzi | ? | - |
? | |
peptidyl-beta-naphthylamide + H2O | derivatives of | Trypanosoma cruzi | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
unstable in the absence of substrate at pH 8.7 | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0805 | - |
N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide | - |
Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay with azocasein | Trypanosoma cruzi |
7 | - |
beta-naphthylamide derivatives as substrate | Trypanosoma cruzi |
9 | - |
4-nitroanilide derivatives as substrate | Trypanosoma cruzi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
unstable in the absence of substrate at 37°C | Trypanosoma cruzi |