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Literature summary for 3.4.22.51 extracted from

  • Alvarez, V.; Parussini, F.; Aslund, L.; Cazzulo, J.J.
    Expression in insect cells of active mature cruzipain from Trypanosoma cruzi, containing its C-terminal domain (2002), Protein Expr. Purif., 26, 467-475.
    View publication on PubMed
Search for more literature for 3.4.22.51

Cloned(Commentary)

Cloned (Comment) Organism
active complete mature enzyme Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 deglycosylated enzyme, SDS-PAGE Trypanosoma cruzi
51000
-
 glycosylated enzyme, SDS-PAGE Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q598P5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trypanosoma cruzi

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma cruzi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84
-
-
 Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information synthetic chromogenic sustrates Trypanosoma cruzi ?
-
 ?
peptidyl-4-nitroanilide + H2O derivatives of Trypanosoma cruzi ?
-
 ?
peptidyl-beta-naphthylamide + H2O derivatives of Trypanosoma cruzi ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay with beta-naphthylamide Trypanosoma cruzi
7.6
-
 assay with 4-nitroanilide Trypanosoma cruzi