Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | separase is abundantly expressed within the cytoplasm of a broad range of human tumor cell lines, including MDA-MB-231 breast carcinoma cells | Homo sapiens | 5737 | - |
membrane | - |
Homo sapiens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
MDA-MB-231 cell | - |
Homo sapiens | - |
General Information | Comment | Organism |
---|---|---|
malfunction | cells depleted of securin or separase display defective acidification of early endosomes and increased membrane recruitment of vacuolar ATPase complexes, mimicking the effect of the specific V-ATPase inhibitor Bafilomycin A1. Securin and separase depletion causes trans-Golgi network and endosome swelling independent of cell cycle. Endosome-mediated receptor degradation and recycling are also significantly impaired by securin and separase depletion, although not receptor internalization or Rab5 activity and autophagy | Homo sapiens |
physiological function | functional role of securin and separase in the modulation of membrane traffic and protein secretion implicating regulation of V-ATPase assembly and function. Separase activity is controlled by securin, i.e. pituitary tumor transforming gene 1, PTTG1, a member of a divergent class of anaphase inhibitors whose proteosomal degradation by the anaphase promoting complex, APC, is required to release separase and allow its activation | Homo sapiens |