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Literature summary for 3.4.22.49 extracted from
- Development and validation of a fluorogenic assay to measure separase enzyme activity (2009), Anal. Biochem., 392, 133-138.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | separase can be activated by incubating immunoglobulin G-Sepharose 6 containing ZZ TEV4-separase with anaphase-initiated Xenopus cytostatic factor to degrade its inhibitor securin at room temperature for 1 h | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in HEK-293T cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| securin | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.293 | - |
acetyl-Asp-Arg-Glu-Ile-Nle-Arg-7-amido-4-methylcoumarin | at 37°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Rad21 + H2O | Homo sapiens | - |
fragments of Rad21 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEL cell | - |
Homo sapiens | - |
| HL-60 cell | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1 week, no significant loss of activity | Homo sapiens |
| 23°C, 48 h, 17% loss of activity | Homo sapiens |
| 4°C, 48 h, 12% loss of activity | Homo sapiens |
| temperature, storage medium, duration, loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-Asp-Arg-Glu-Ile-Nle-Arg-7-amido-4-methylcoumarin + H2O | acetyl-Asp-Arg-Glu-Ile-Nle-Arg is the Rad21-peptidyl | Homo sapiens | acetyl-Asp-Arg-Glu-Ile-Nle-Arg + 7-amino-4-methylcoumarin | - |
? | |
| additional information | does not cleave Leu-Glu-His-Asp-CHO-7-amido-4-methylcoumarin | Homo sapiens | ? | - |
? | |
| Rad21 + H2O | - |
Homo sapiens | fragments of Rad21 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
no appreciable activity is observed at 4°C | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Homo sapiens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | separase is overexpressed and mislocalized in a wide range of human cancers, including breast, prostate, and osteosarcoma | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | separase plays a pivotal role in the separation of sister chromatids at anaphase by cleaving its substrate cohesin Rad21 | Homo sapiens |
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