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Literature summary for 3.4.22.48 extracted from

  • Kalinska, M.; Kantyka, T.; Greenbaum, D.C.; Larsen, K.S.; Wladyka, B.; Jabaiah, A.; Bogyo, M.; Daugherty, P.S.; Wysocka, M.; Jaros, M.; Lesner, A.; Rolka, K.; Schaschke, N.; Stennicke, H.; Dubin, A.; Potempa, J.; Dubin, G.
    Substrate specificity of Staphylococcus aureus cysteine proteases - Staphopains A, B and C (2012), Biochimie, 94, 318-327.
    View publication on PubMed
Search for more literature for 3.4.22.48

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.0076
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.014
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
0.154
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.1639
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.271
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
0.385
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
0.5871
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.612
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide + H2O
-
 Staphylococcus aureus ?
-
 ?
2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide + H2O
-
 Staphylococcus aureus ?
-
 ?
2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide + H2O
-
 Staphylococcus aureus ?
-
 ?

Synonyms

Synonyms Comment Organism
staphopain A
-
 Staphylococcus aureus
staphopain B
-
 Staphylococcus aureus
staphopain C
-
 Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
0.0112
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.012
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.023
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
0.032
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.056
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.089
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
0.716
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
0.886
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, value below 0.1, staphopain A Staphylococcus aureus
0.1
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, value below 0.1, staphopain C Staphylococcus aureus
0.1
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, value below 0.1, staphopain C Staphylococcus aureus
0.7
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
62.4
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
118
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
127.8
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus