Cloned (Comment) | Organism |
---|---|
ssp and scp operons, genomic organization of ssp and scp genes, overview | Staphylococcus epidermidis |
ssp and scp operons, genomic organization of ssp and scp genes, overview | Staphylococcus warneri |
ssp and scp operons, genomic organization of ssp and scp genes, overview, overexpression of scp operon genes in Escherichia coli | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
crystal structure determination of staphopain A in complex with inhibitor E64, folding pattern | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | inactivation of gene sspA by transposon insertion exertes a polar effect on genes sspB and sspC expression, the inactivation of all three genes results in attenuation of virulence | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E64 | ScpA-inhibitor binding structure | Staphylococcus aureus | |
additional information | staphostin homologue genes are probably encoding enzyme inhibitors | Staphylococcus epidermidis | |
additional information | staphostin homologue genes are probably encoding enzyme inhibitors | Staphylococcus warneri | |
staphostatin A | i.e. ScpB, intracellular, endogenous specific inhibitor of ScpA forming noncovalent complexes, structure determination, slight cleaving of the inhibitor by the enzyme | Staphylococcus aureus | |
staphostatin B | i.e. SspC, intracellular, endogenous specific inhibitor of SspB forming noncovalent complexes, structure determination, slight cleaving of the inhibitor by the enzyme | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted as zymogen | Staphylococcus aureus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kininogen + H2O | Staphylococcus aureus | activation of human protein by SspA, kinin generation is responsible for infection associated pain and endema | kinin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus epidermidis | - |
- |
- |
Staphylococcus warneri | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme staphopain B is synthesized as preproenzyme, the prefragment is cleaved resulting in the proenzyme, after secretion the proenzyme is proteolytically activated to the mature enzyme | Staphylococcus aureus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate | active site structure and topology of ScpA | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha1-proteinase inhibitor + H2O | human protein, inactivation by SspA | Staphylococcus aureus | ? | - |
? | |
Gelatin + H2O | staphopain B | Staphylococcus aureus | ? | - |
? | |
kininogen + H2O | activation of human protein by SspA, kinin generation is responsible for infection associated pain and endema | Staphylococcus aureus | kinin + ? | - |
? | |
kininogen + H2O | human protein, activation by SspA | Staphylococcus aureus | kinin + ? | - |
? | |
additional information | staphopains A and B are cysteine proteases, staphopain shows no activity with casein | Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCPA | - |
Staphylococcus aureus |
SspB | - |
Staphylococcus aureus |
staphopain A | - |
Staphylococcus aureus |
staphopain B | - |
Staphylococcus aureus |