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Literature summary for 3.4.22.48 extracted from
- Staphylopain (1998), Handbook of Proteolytic Enzymes (Barrett, A. ; Rawlings, N. D. ; Woessner, J. F. eds. ), , 669-671.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| reducing thiol compounds | dependent on | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Staphylococcus aureus |  |
| alpha2-Macroglobulin | from human | Staphylococcus aureus | |
| E-64 | stochiometrical and irreversible inhibition | Staphylococcus aureus | |
| Hg2+ | - |
Staphylococcus aureus | |
| additional information | no inhibition by human kininogens and cystatin C | Staphylococcus aureus | |
| phosphorylated cystatin alpha | from rat skin | Staphylococcus aureus | |
| T-Kininogen | from rat | Staphylococcus aureus | |
| Zn2+ | - |
Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide | pH 8.0-8.8 | Staphylococcus aureus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | culture supernatant | Staphylococcus aureus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by changes in ionic strength by changing NaCl concentration | Staphylococcus aureus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13000 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptide + H2O | Staphylococcus aureus | broad specificity | peptide proteolytically cleaved into fragments or single amino acids | - |
? | |
| protein + H2O | Staphylococcus aureus | broad specificity | protein proteolytically cleaved into peptide fragments | - |
? | |
| protein + H2O | Staphylococcus aureus | enzyme may play a role in growth regulation | protein proteolytically cleaved into peptide fragments | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
enzyme might be inducible by elastin in vivo | - |
| Staphylococcus aureus | - |
V8 | - |
| Staphylococcus aureus V8 | - |
V8 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native from culture supernatant | Staphylococcus aureus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Staphylococcus aureus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alcohol dehydrogenase + H2O | - |
Staphylococcus aureus | alcohol dehydrogenase proteolytically cleaved into peptide fragments | - |
? | |
| alcohol dehydrogenase + H2O | - |
Staphylococcus aureus V8 | alcohol dehydrogenase proteolytically cleaved into peptide fragments | - |
? | |
| benzyl-Tyr-OEt + H2O | - |
Staphylococcus aureus | ? | - |
? | |
| casein + H2O | - |
Staphylococcus aureus | casein proteolytically cleaved into peptide fragments | - |
? | |
| casein + H2O | - |
Staphylococcus aureus V8 | casein proteolytically cleaved into peptide fragments | - |
? | |
| elastin + H2O | insoluble substrate | Staphylococcus aureus | elastin proteolytically cleaved into peptide fragments | - |
? | |
| hemoglobin + H2O | - |
Staphylococcus aureus | hemoglobin proteolytically cleaved into peptide fragments | - |
? | |
| hemoglobin + H2O | - |
Staphylococcus aureus V8 | hemoglobin proteolytically cleaved into peptide fragments | - |
? | |
| N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide + H2O | - |
Staphylococcus aureus | N-benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline | - |
? | |
| peptide + H2O | broad specificity | Staphylococcus aureus | amino acids | - |
? | |
| peptide + H2O | broad specificity | Staphylococcus aureus V8 | amino acids | - |
? | |
| peptide + H2O | broad specificity | Staphylococcus aureus | peptide proteolytically cleaved into fragments or single amino acids | - |
? | |
| protein + H2O | broad specificity | Staphylococcus aureus | peptide fragments | - |
? | |
| protein + H2O | broad specificity | Staphylococcus aureus V8 | peptide fragments | - |
? | |
| protein + H2O | broad specificity | Staphylococcus aureus | protein proteolytically cleaved into peptide fragments | - |
? | |
| protein + H2O | enzyme may play a role in growth regulation | Staphylococcus aureus | protein proteolytically cleaved into peptide fragments | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 13000, SDS-PAGE | Staphylococcus aureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide | pH 8.0-8.8 | Staphylococcus aureus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 9.4 | Staphylococcus aureus |
| 6.5 | - |
substrate insoluble elastin | Staphylococcus aureus |
| 7.5 | - |
substrate benzyl-Tyr-OEt | Staphylococcus aureus |
| 8 | 8.8 | substrate casein or hemoglobin | Staphylococcus aureus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00052 | - |
E-64 | pH 8.0-8.8 | Staphylococcus aureus | |
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