Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)LysE cells | Foot-and-mouth disease virus |
Crystallization (Comment) | Organism |
---|---|
isoform sLbpro in complex with inhibitor E-64-R-P-NH2, hanging drop vapor diffusion method, using 0.1 M sodium acetate pH 4.8, 0.9 M NaH2PO4 and 1.2 M K2HPO4 | Foot-and-mouth disease virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CA074 | - |
Foot-and-mouth disease virus | |
E64-R-P-NH2 | - |
Foot-and-mouth disease virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
eukaryotic initiation factor eIF4G + H2O | Foot-and-mouth disease virus | in intermolecular cleavage on polyprotein substrates, isoform Lbpro is unaffected by P1 or P1' substitutions and processes a substrate containing nine eIF4GI cleavage site residues whereas isoform sLbpro fails to cleave the eIF4GI containing substrate and cleaves appreciably more slowly on mutated substrates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Foot-and-mouth disease virus | P03305 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eukaryotic initiation factor eIF4G + H2O | in intermolecular cleavage on polyprotein substrates, isoform Lbpro is unaffected by P1 or P1' substitutions and processes a substrate containing nine eIF4GI cleavage site residues whereas isoform sLbpro fails to cleave the eIF4GI containing substrate and cleaves appreciably more slowly on mutated substrates | Foot-and-mouth disease virus | ? | - |
? | |
SFANLGRTTL + H2O | poor substrate for both isoforms Lbpro and sLbpro | Foot-and-mouth disease virus | SFANLG + RTTL | - |
? | |
VQRKLGAAGQ + H2O | isoform sLbpro cleaves this substrate better than isoform Lbpro | Foot-and-mouth disease virus | VQRKLG + AAGQ | - |
? | |
VQRKLGRAGQ + H2O | isoform sLbpro cleaves this substrate better than isoform Lbpro | Foot-and-mouth disease virus | VQRKLG + RAGQ | - |
? | |
VQRKLKGAGQ + H2O | isoform sLbpro cleaves this substrate better than isoform Lbpro | Foot-and-mouth disease virus | VQRKLK + GAGQ | - |
? | |
VQRKLKRAGQ + H2O | isoform sLbpro cleaves this substrate better than isoform Lbpro | Foot-and-mouth disease virus | VQRKLK + RAGQ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lbpro | isoform | Foot-and-mouth disease virus |
Leader proteinase | - |
Foot-and-mouth disease virus |
Lpro | - |
Foot-and-mouth disease virus |
sLbpro | during infection, isoform Lbpro removes six residues from its own C-terminus, generating sLbpro | Foot-and-mouth disease virus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00003 | - |
E64-R-P-NH2 | isoform Lbpro, pH and temperature not specified in the publication | Foot-and-mouth disease virus | |
0.0116 | - |
CA074 | isoform Lbpro, pH and temperature not specified in the publication | Foot-and-mouth disease virus |