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Literature summary for 3.4.22.45 extracted from
- Expression, purification and functional characterization of recombinant Zucchini yellow mosaic virus HC-Pro (2011), Protein Expr. Purif., 75, 40-45.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged or MBP-fused wild-type enzyme and of MBP-fused mutant HC-ProFINK protein in Escherichia coli strain BL21 (DE3) | zucchini yellow mosaic virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a point mutation in the highly conserved FRNK box produces the HC-ProFINK protein that shows no sRNA binding | zucchini yellow mosaic virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| zucchini yellow mosaic virus | A1DU45 | polyprotein, sequence ID of mutant HC-ProFINK protein is A1DU46; ZYMV | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged or MBP-fused wild-type enzyme and MBP-fused mutant HC-ProFINK protein from Escherichia coli strain BL21 (DE3) by affinity chromatography | zucchini yellow mosaic virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | analysis of RNA-binding activity of HC-Pro by EMSA, overview | zucchini yellow mosaic virus | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HC-Pro | - |
zucchini yellow mosaic virus |
| helper component-proteinase | - |
zucchini yellow mosaic virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
sRNA binding assay at | zucchini yellow mosaic virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
sRNA binding assay at | zucchini yellow mosaic virus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | a point mutation in the highly conserved FRNK box produces the HC-ProFINK protein that shows no sRNA binding | zucchini yellow mosaic virus |
| physiological function | HC-Pro is a helper component-proteinase which acts as a multifunctional protein in the potyviral life cycle. Apart from its proteolytic activity, HC-Pro has the capacity to bind duplex small RNAs | zucchini yellow mosaic virus |
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Release 2023.1 (January 2023)
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