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Literature summary for 3.4.22.45 extracted from

  • Yambao, M.L.; Yagihashi, H.; Sekiguchi, H.; Sekiguchi, T.; Sasaki, T.; Sato, M.; Atsumi, G.; Tacahashi, Y.; Nakahara, K.S.; Uyeda, I.
    Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean (2008), Arch. Virol., 153, 105-115.
    View publication on PubMed
Search for more literature for 3.4.22.45

Application

Application Comment Organism
agriculture involvement of helper component protease HC-Pro in necrotic symptom expression in broad bean Vicia faba indicated, functional importance for domains of helper component protease HC-Pro determined clover yellow vein virus

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant protein, expressed in Escherichia coli clover yellow vein virus

Protein Variants

Protein Variants Comment Organism
D193Y mutants of helper component protease HC-Pro of clover yellow vein virus, generated by site-directed mutagenesis or by PCR-based primer extension mutagenesis, revertant of D193Y also created, single mutant alone does not induce lethal necrosis clover yellow vein virus
I33 mutants of helper component protease HC-Pro of clover yellow vein virus generated by site-directed mutagenesis or by PCR-based primer extension mutagenesis clover yellow vein virus
R51I mutants of helper component protease HC-Pro of clover yellow vein virus generated by site-directed mutagenesis or by PCR-based primer extension mutagenesis clover yellow vein virus
T27I mutants of helper component protease HC-Pro of clover yellow vein virus generated by site-directed mutagenesis or by PCR-based primer extension mutagenesis, revertant of T27I also created, single mutant alone does not induce lethal necrosis but retains ability to induce necrotic symptoms clover yellow vein virus
T27I/D193Y double mutant clover yellow vein virus

Organism

Organism UniProt Comment Textmining
clover yellow vein virus
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 potyvirus, clover yellow vein virus no. 30, induction of lethal necrosis in the broad bean Vicia faba
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Purification (Commentary)

Purification (Comment) Organism
recombinant protein clover yellow vein virus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 involvement of helper component protease HC-Pro in necrotic symptom expression in broad bean Vicia faba indicated, characterization of a spontaneous mutant (MM) that does not induce lethal necrosis on broad bean assigned to helper component protease HC-Pro, mutations T27I and D193Y assigned to putative zinc finger domain and to the N-terminal RNA binding domain, respectively, RNA-silencing suppression activity of helper component protease HC-Pro in Nicotiana benthamiana shown to be weakened by both mutations, T27I and D193Y, respectively clover yellow vein virus

Synonyms

Synonyms Comment Organism
HC-Pro protein
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 clover yellow vein virus