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Literature summary for 3.4.22.44 extracted from

  • Parks, T.D.; Howard, E.D.; Wolpert, T.J.; Arp, D.J.; Dougherty, W.G.
    Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form (1995), Virology, 210, 194-201.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
tobacco etch virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg 27 kDa full length protease tobacco etch virus
0.271
-
Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg truncated protease tobacco etch virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nuclear inclusion protein a + H2O tobacco etch virus
-
?
-
?
nuclear inclusion protein a + H2O tobacco etch virus TEV
-
?
-
?

Organism

Organism UniProt Comment Textmining
tobacco etch virus
-
TEV
-
tobacco etch virus TEV
-
TEV
-

Purification (Commentary)

Purification (Comment) Organism
-
tobacco etch virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nuclear inclusion protein a + H2O
-
tobacco etch virus ?
-
?
nuclear inclusion protein a + H2O
-
tobacco etch virus TEV ?
-
?
Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg + H2O
-
tobacco etch virus Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln + Ser-Gly-Thr-Val-Asp-Arg-Arg
-
?
Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg + H2O
-
tobacco etch virus TEV Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln + Ser-Gly-Thr-Val-Asp-Arg-Arg
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.033
-
Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg truncated protease tobacco etch virus
0.18
-
Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg 27 kDa full length protease tobacco etch virus