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Literature summary for 3.4.22.40 extracted from
- Neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids (2009), J. Biol. Chem., 284, 12829-12836.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
6 * 48000, SDS-PAGE | Homo sapiens |
| 48000 | - |
6 * 48000, SDS-PAGE | Rattus norvegicus |
| 280000 | - |
gel filtration | Homo sapiens |
| 280000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bleomycin + H2O | Homo sapiens | - |
? | - |
? |  |
| bleomycin + H2O | Rattus norvegicus | - |
? | - |
? | |
| deiminated filaggrin + H2O | Homo sapiens | neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids | ? | - |
? | |
| deiminated filaggrin + H2O | Rattus norvegicus | neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 4300fold from newborn rat epidermis by gel filtration, and two steps of anion exchange chromatography | Homo sapiens |
| native enzyme 4300fold from newborn rat epidermis by gel filtration, and two steps of anion exchange chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | from newborn rats | Rattus norvegicus | - |
| epidermis | the enzyme co-localizes with fillagrin in the granular layer, immunohistochemic analysis | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bleomycin + H2O | - |
Homo sapiens | ? | - |
? | |
| bleomycin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| bleomycin + H2O | from rat | Rattus norvegicus | ? | - |
? | |
| citrulline-beta-naphthylamide + H2O | - |
Homo sapiens | citrulline + beta-naphthylamine | - |
? | |
| citrulline-beta-naphthylamide + H2O | - |
Rattus norvegicus | citrulline + beta-naphthylamine | - |
? | |
| deiminated filaggrin + H2O | neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids | Homo sapiens | ? | - |
? | |
| deiminated filaggrin + H2O | neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids | Rattus norvegicus | ? | - |
? | |
| deiminated filaggrin + H2O | recombinant human filaggrin is a component of the cornified cell envelope and the precursor of free amino acids acting as a natural moisturizing factor in the stratum corneum. Profilaggrin is synthesized as a large, extremely insoluble phosphoprotein. Deimination is critical for the degradation of filaggrin into free amino acids, deimination of filaggrin is catalyzed by murine PAD3 | Homo sapiens | ? | - |
? | |
| deiminated filaggrin + H2O | recombinant human filaggrin is a component of the cornified cell envelope and the precursor of free amino acids acting as a natural moisturizing factor in the stratum corneum. Profilaggrin is synthesized as a large, extremely insoluble phosphoprotein. Deimination is critical for the degradation of filaggrin into free amino acids, deimination of filaggrin is catalyzed by murine PAD3 | Rattus norvegicus | ? | - |
? | |
| additional information | bleomycin hydrolase is a neutral cysteine protease, the enzyme releases various amino acids except Pro from beta-naphthylamide derivatives and hydrolyzed citrulline-beta-naphthylamide most effectively, overview | Homo sapiens | ? | - |
? | |
| additional information | bleomycin hydrolase is a neutral cysteine protease, the enzyme releases various amino acids except Pro from beta-naphthylamide derivatives and hydrolyzed citrulline-beta-naphthylamide most effectively, overview | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 48000, SDS-PAGE | Homo sapiens |
| hexamer | 6 * 48000, SDS-PAGE | Rattus norvegicus |
| More | trypsin-mediated peptide mapping, overview | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| citrulline aminopeptidase | - |
Homo sapiens |
| citrulline aminopeptidase | - |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is most active at neutral pH | Homo sapiens |
| additional information | - |
the enzyme is most active at neutral pH | Rattus norvegicus |
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