Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Brij 35 | 245.48% relative activity at 0.01% (v/v) | Paralichthys olivaceus | |
SDS | 135.84% relative activity at 0.01% (w/v) | Paralichthys olivaceus | |
Triton X-100 | 218.51% relative activity at 0.05% (v/v) | Paralichthys olivaceus | |
Tween 20 | 218.04% relative activity at 0.05% (v/v) | Paralichthys olivaceus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain DH5alphaMCR using the pGEX-4T-1 expression vector system | Paralichthys olivaceus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | 98.91% inhibition at 0.1 mM | Paralichthys olivaceus | |
Aprotinin | 25.08% inhibition at 0.1 mM | Paralichthys olivaceus | |
chymostatin | 44.58% inhibition at 0.1 mM | Paralichthys olivaceus | |
Co2+ | 5.09% residual activity at 5 mM | Paralichthys olivaceus | |
Cu2+ | 24.86% residual activity at 5 mM | Paralichthys olivaceus | |
E-64 | 60.15% inhibition at 0.5 mM | Paralichthys olivaceus | |
EDTA | 3.72% inhibition at 0.1 mM | Paralichthys olivaceus | |
Hg2+ | 25.27% residual activity at 5 mM | Paralichthys olivaceus | |
leupeptin | complete inhibition at 0.1 mM | Paralichthys olivaceus | |
additional information | not inhibited by EGTA and 1,10-phenanthroline | Paralichthys olivaceus | |
pepstatin A | 17.29% inhibition at 0.1 mM | Paralichthys olivaceus | |
phenylmethylsulfonyl fluoride | 53.68% inhibition at 0.1 mM | Paralichthys olivaceus | |
SDS | 77.28% residual activity at 0.05% (w/v) | Paralichthys olivaceus | |
Zn2+ | 47.5% residual activity at 5 mM | Paralichthys olivaceus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 390.04% relative activity at 1 mM | Paralichthys olivaceus | |
K+ | 332.4%7 relative activity at 1 mM | Paralichthys olivaceus | |
Mg2+ | 125.02% relative activity at 5 mM | Paralichthys olivaceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
His6-tagged fusion protein, SDS-PAGE | Paralichthys olivaceus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
collagen I + H2O | Paralichthys olivaceus | - |
? | - |
? | |
collagen II + H2O | Paralichthys olivaceus | - |
? | - |
? | |
collagen III + H2O | Paralichthys olivaceus | - |
? | - |
? | |
Collagen IV + H2O | Paralichthys olivaceus | - |
? | - |
? | |
collagen VI + H2O | Paralichthys olivaceus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paralichthys olivaceus | B2Z447 | olive flounder | - |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Paralichthys olivaceus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone | - |
Paralichthys olivaceus | - |
bone marrow | high levels of expression | Paralichthys olivaceus | - |
brain | - |
Paralichthys olivaceus | - |
esophagus | - |
Paralichthys olivaceus | - |
eye | - |
Paralichthys olivaceus | - |
gill | - |
Paralichthys olivaceus | - |
head kidney | - |
Paralichthys olivaceus | - |
heart | - |
Paralichthys olivaceus | - |
intestine | - |
Paralichthys olivaceus | - |
liver | - |
Paralichthys olivaceus | - |
muscle | - |
Paralichthys olivaceus | - |
pyloric cecum | - |
Paralichthys olivaceus | - |
spleen | high levels of expression | Paralichthys olivaceus | - |
stomach | - |
Paralichthys olivaceus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | 31.29% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | 47.92% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O | 89.78% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | 100% activity | Paralichthys olivaceus | benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O | 23.91% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | benzyloxycarbonyl-Leu-Leu-Glu + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | 71.17% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
collagen I + H2O | - |
Paralichthys olivaceus | ? | - |
? | |
collagen II + H2O | - |
Paralichthys olivaceus | ? | - |
? | |
collagen III + H2O | - |
Paralichthys olivaceus | ? | - |
? | |
Collagen IV + H2O | - |
Paralichthys olivaceus | ? | - |
? | |
collagen VI + H2O | - |
Paralichthys olivaceus | ? | - |
? | |
additional information | cathepsin K is not able to hydrolyze protein substrates such as azocasein, fibrinogen, and bovine serum albumin | Paralichthys olivaceus | ? | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O | 24.13% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Ile-Ala-7-amido-4-methylcoumarin + H2O | 17.34% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | N-succinyl-Ile-Ala + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | 25.03% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O | 31.57% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | Paralichthys olivaceus | N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ctk | - |
Paralichthys olivaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paralichthys olivaceus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Paralichthys olivaceus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
the activity of cathepsin K is profoundly reduced at pH values of 6.0 (lysosomal pH) and below | Paralichthys olivaceus |
Organism | Comment | Expression |
---|---|---|
Paralichthys olivaceus | CtK expression is significantly increased in the muscle and gill at 3-24 h post-injection with bacterial lipopolysaccharide | up |
General Information | Comment | Organism |
---|---|---|
physiological function | cathepsin K may play a role in the immune system of fish skin and muscle, in addition to its principal bone-specific function as a collagenolytic enzyme | Paralichthys olivaceus |