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Literature summary for 3.4.22.38 extracted from

  • Je, J.E.; Ahn, S.J.; Kim, N.Y.; Seo, J.S.; Kim, M.S.; Park, N.G.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Molecular cloning, expression analysis and enzymatic characterization of cathepsin K from olive flounder (Paralichthys olivaceus) (2009), Comp. Biochem. Physiol. A, 154, 474-485.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Brij 35 245.48% relative activity at 0.01% (v/v) Paralichthys olivaceus
SDS 135.84% relative activity at 0.01% (w/v) Paralichthys olivaceus
Triton X-100 218.51% relative activity at 0.05% (v/v) Paralichthys olivaceus
Tween 20 218.04% relative activity at 0.05% (v/v) Paralichthys olivaceus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain DH5alphaMCR using the pGEX-4T-1 expression vector system Paralichthys olivaceus

Inhibitors

Inhibitors Comment Organism Structure
antipain 98.91% inhibition at 0.1 mM Paralichthys olivaceus
Aprotinin 25.08% inhibition at 0.1 mM Paralichthys olivaceus
chymostatin 44.58% inhibition at 0.1 mM Paralichthys olivaceus
Co2+ 5.09% residual activity at 5 mM Paralichthys olivaceus
Cu2+ 24.86% residual activity at 5 mM Paralichthys olivaceus
E-64 60.15% inhibition at 0.5 mM Paralichthys olivaceus
EDTA 3.72% inhibition at 0.1 mM Paralichthys olivaceus
Hg2+ 25.27% residual activity at 5 mM Paralichthys olivaceus
leupeptin complete inhibition at 0.1 mM Paralichthys olivaceus
additional information not inhibited by EGTA and 1,10-phenanthroline Paralichthys olivaceus
pepstatin A 17.29% inhibition at 0.1 mM Paralichthys olivaceus
phenylmethylsulfonyl fluoride 53.68% inhibition at 0.1 mM Paralichthys olivaceus
SDS 77.28% residual activity at 0.05% (w/v) Paralichthys olivaceus
Zn2+ 47.5% residual activity at 5 mM Paralichthys olivaceus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 390.04% relative activity at 1 mM Paralichthys olivaceus
K+ 332.4%7 relative activity at 1 mM Paralichthys olivaceus
Mg2+ 125.02% relative activity at 5 mM Paralichthys olivaceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
His6-tagged fusion protein, SDS-PAGE Paralichthys olivaceus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
collagen I + H2O Paralichthys olivaceus
-
?
-
?
collagen II + H2O Paralichthys olivaceus
-
?
-
?
collagen III + H2O Paralichthys olivaceus
-
?
-
?
Collagen IV + H2O Paralichthys olivaceus
-
?
-
?
collagen VI + H2O Paralichthys olivaceus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Paralichthys olivaceus B2Z447 olive flounder
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Paralichthys olivaceus

Source Tissue

Source Tissue Comment Organism Textmining
bone
-
Paralichthys olivaceus
-
bone marrow high levels of expression Paralichthys olivaceus
-
brain
-
Paralichthys olivaceus
-
esophagus
-
Paralichthys olivaceus
-
eye
-
Paralichthys olivaceus
-
gill
-
Paralichthys olivaceus
-
head kidney
-
Paralichthys olivaceus
-
heart
-
Paralichthys olivaceus
-
intestine
-
Paralichthys olivaceus
-
liver
-
Paralichthys olivaceus
-
muscle
-
Paralichthys olivaceus
-
pyloric cecum
-
Paralichthys olivaceus
-
spleen high levels of expression Paralichthys olivaceus
-
stomach
-
Paralichthys olivaceus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 31.29% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O 47.92% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O 89.78% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O 100% activity Paralichthys olivaceus benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O 23.91% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus benzyloxycarbonyl-Leu-Leu-Glu + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O 71.17% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
collagen I + H2O
-
Paralichthys olivaceus ?
-
?
collagen II + H2O
-
Paralichthys olivaceus ?
-
?
collagen III + H2O
-
Paralichthys olivaceus ?
-
?
Collagen IV + H2O
-
Paralichthys olivaceus ?
-
?
collagen VI + H2O
-
Paralichthys olivaceus ?
-
?
additional information cathepsin K is not able to hydrolyze protein substrates such as azocasein, fibrinogen, and bovine serum albumin Paralichthys olivaceus ?
-
?
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O 24.13% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
?
N-succinyl-Ile-Ala-7-amido-4-methylcoumarin + H2O 17.34% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus N-succinyl-Ile-Ala + 7-amino-4-methylcoumarin
-
?
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 25.03% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O 31.57% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
Ctk
-
Paralichthys olivaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Paralichthys olivaceus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
Paralichthys olivaceus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
the activity of cathepsin K is profoundly reduced at pH values of 6.0 (lysosomal pH) and below Paralichthys olivaceus

Expression

Organism Comment Expression
Paralichthys olivaceus CtK expression is significantly increased in the muscle and gill at 3-24 h post-injection with bacterial lipopolysaccharide up

General Information

General Information Comment Organism
physiological function cathepsin K may play a role in the immune system of fish skin and muscle, in addition to its principal bone-specific function as a collagenolytic enzyme Paralichthys olivaceus