Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chondroitin 4-sulfate | 18.5 kDa and 37 kDa fractions, the unique triple-helical collagen-degrading activity of cathepsin K depends on the formation of complexes with bone-or cartilage-resident glycosaminoglycans, such as chondroitin 4-sulfate, structure of the complex involving Asp6, Arg8, Lys9, Lys10, Ile171, Glu172, and Asn190 for interaction, the binding sites with chondroitin 4-sulfate are located in the R-domain of cathepsin K and are distant from its active site, detailed overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression as the proenzyme in Pichia pastoris | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified and activated, recombinant cathepsin K in complex with chondroitin sulfate in presence or absence of inhibitor E64, which prevents autocatalytic cleavage of cathepsin K, 25 mg/ml enzyme-inhibitor complex of ratio 1:1 in 50 mM sodium acetate buffer, pH 5.5, hanging drop vapor diffusion method, mixing with an equal volume of reservoir solution containing 30% v/v 2-methyl-2,4-pentanediol, 0.1 M sodium acetate buffer, pH 4.5, and 20 mM calcium chloride, macroseeding, X-ray diffraction structure determination and analysis at 1.8 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E64 | i.e. L-3-carboxy-trans-2,3-epoxypropionyl-leucylamido-(4-guanidino)butane, inhibitor-enzyme complex structure 9in presence of different chondroitin 4-sulfate fractions, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | cathepsin K is the major collagenolytic enzyme produced by bone-resorbing osteoclasts | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the recombinant proenzyme is activated by cleavage through pepsin | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
osteoclast | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cathepsin K is the major collagenolytic enzyme produced by bone-resorbing osteoclasts | Homo sapiens | ? | - |
? |