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Literature summary for 3.4.22.38 extracted from

  • Tada, S.; Tsutsumi, K.; Ishihara, H.; Suzuki, K.; Gohda, K.; Teno, N.
    Species differences between human and rat in the substrate specificity of cathepsin K (2008), J. Biochem., 144, 499-506.
    View publication on PubMed
Search for more literature for 3.4.22.38

Activating Compound

Activating Compound Comment Organism Structure
additional information procathepsin K is activated by incubation at 37°C in the presence of DTT and dextran sulfate at final concentrations of 1 mM and 100 mg/ml, respectively Rattus norvegicus
additional information procathepsin K is activated by incubation at 37°C in the presence of DTT and dextran sulfate at final concentrations of 1 mM and 100 mg/ml, respectively Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloning from a bone cDNA library, expression in Tn cells Homo sapiens
recombinant expression in Tn cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information site-directed mutagenesis of the rat residues Ser and Val to the correspondent Ala and Leu, respectively, of the human sequence causes a loss of the P2 Hyp specificity, overview Homo sapiens
S134A site-directed mutagenesis of the rat residue Ser to the correspondent Ala of the human sequence, the mutant looses the P2 Hyp specificity Rattus norvegicus
S134A/V160L site-directed mutagenesis of the rat residues Ser and Val to the correspondent Ala and Leu, respectively, of the human sequence, the mutant looses the P2 Hyp specificity Rattus norvegicus
V160L site-directed mutagenesis of the rat residue Val to the correspondent Leu of the human sequence, the mutant looses the P2 Hyp specificity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant mutant S134A/V160L Rattus norvegicus
0.0075
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant enzyme Homo sapiens
0.016
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant mutant S134A Rattus norvegicus
0.026
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant mutant V160L Rattus norvegicus
0.293
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant wild-type enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
collagen I + H2O Rattus norvegicus
-
 ?
-
 ?
collagen I + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P43235
-
-
Rattus norvegicus O35186
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-Gly-Pro-Hyp-Gly-Phe(NO2)-Gly-OH + H2O
-
 Rattus norvegicus ?
-
 ?
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide + H2O
-
 Rattus norvegicus carbobenzyloxy-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens carbobenzyloxy-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
collagen I + H2O
-
 Rattus norvegicus ?
-
 ?
collagen I + H2O
-
 Homo sapiens ?
-
 ?
collagen I + H2O rat tail type I collagen Rattus norvegicus ?
-
 ?
collagen I + H2O rat tail type I collagen Homo sapiens ?
-
 ?
additional information human cathepsin K preferentially hydrolyses the substrates with Pro at the P2 position, substrate specificity compared to the rat enzyme, overview Homo sapiens ?
-
 ?
additional information rat cathepsin K preferentially hydrolyses the substrates at the P2 Hyp position, substrate specificity compared to the human enzyme, overview Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
More the human sequence contains Asp and Tyr at S3 positions 61 and 67, and residues Met, Ala, Leu, Ala and Leu at S2 positions 68, 134, 160, 163, and 209, respectively, comparison with the rat enzyme Homo sapiens
More the human sequence contains Tyr at S3 positions 61 and 67, and residues Met, Ser, Val, Ala, and Leu at S2 positions 68, 134, 160, 163, and 209, respectively, comparison with the human enzyme Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37 assay at, substrate dependently Rattus norvegicus
25 37 assay at, substrate dependently Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.1
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant mutant S134A/V160L Rattus norvegicus
46.3
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant mutant V160L Rattus norvegicus
56.1
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant enzyme Homo sapiens
95.7
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant wild-type enzyme Rattus norvegicus
97.5
-
 carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide pH 7.0, 37°C, recombinant mutant S134A Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Rattus norvegicus
7
-
 assay at Homo sapiens