Application | Comment | Organism |
---|---|---|
medicine | cathepsin K is shown to play an essential role in rheumatoid arthritis, osteoarthritis, atherosclerosis, lung inflammation, amyloidosis, gaucher disease and obesity | Homo sapiens |
medicine | one genetic disorder is traced to cathepsin K: peridontopathia. Osteoblasts from these patients accumulate undigested collagen fibrils. Nonsense, missense and stop codon mutations have been shown in patients | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
the overall organization of the catalytic site, which consists of two domains folds together to give a V-shaped active site cleft configuration. The central helix is the most prominent feature of the left domain, whereas the right domain is mostly dominated by beta-barrel motifs (5-6 strands). The active site lies at the interface between the two domains | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y67L/L205A | 10fold lower interaction with inhibitor stefin B (Ki: 0.0000000008) suggesting that Tyr67 and Leu205 are critical for interaction with stefin B. Inhibitor cystatin C Ki: 0.0000000087, inhibitor L-kininogen Ki: 0.0000000135, inhibitor H-kininogen Ki: 0.0000000145 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cystatin C | natural inhibitor of cathepsin K, reversible, tight-binding competitive inhibitor | Homo sapiens | |
H-kininogen | natural inhibitor of cathepsin K, reversible, tight-binding competitive inhibitor | Homo sapiens | |
L-kininogen | natural inhibitor of cathepsin K, reversible, tight-binding competitive inhibitor | Homo sapiens | |
Stefin B | natural inhibitor of cathepsin K, reversible, tight-binding competitive inhibitor | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
- |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | one of the two putative N-linked glycosylation sites is located in the propeptide and the other is in the mature protease | Homo sapiens |
additional information | protein is synthesized as a pre-proprotein | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-His-Pro-Gly-Gly-Pro-Gln-EDDnp + H2O | cathepsin k is unusual among the cysteine cathepsins since it can cleave substrates with Pro in the P2 position. A specific peptide substrate for cathepsin K is developed (kcat/Km: 426000/Msec) that is resistant to proteolysis by cathepsin B, L, S, F, H,V and the serine proteases (cathepsin G, chymotrypsin and leukocyte elastase) | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cathepsin K | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000000008 | - |
Stefin B | Y67L/L205A mutant cathepsin K | Homo sapiens | |
0.0000000041 | - |
cystatin C | - |
Homo sapiens | |
0.0000000049 | - |
L-kininogen | - |
Homo sapiens | |
0.0000000054 | - |
H-kininogen | - |
Homo sapiens | |
0.0000000087 | - |
cystatin C | Y67L/L205A mutant cathepsin K | Homo sapiens | |
0.0000000124 | - |
Stefin B | wild-type cathepsin K | Homo sapiens | |
0.0000000135 | - |
L-kininogen | Y67L/L205A mutant cathepsin K | Homo sapiens | |
0.0000000145 | - |
H-kininogen | Y67L/L205A mutant cathepsin K | Homo sapiens |