Literature summary for 3.4.22.38 extracted from

Bossard, M.J.; Tomaszek, T.A.; Thompson, S.K.; Amegadzie, B.Y.; Hanning, C.R.; Jones, C.J.; Kurdyla, J.T.; McNulty, D.E.; Drake, F.H.; Gowen, M.; Levy, M.A.
Proteolytic activity of human osteoclast cathepsin K. Expression, purification, activation and substrate identification (1996), J. Biol. Chem., 271, 12517-12524.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in baculovirus-infected SF21 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
leupeptin	-	Homo sapiens
additional information	not inhibitory are pepstatin, phenylmethylsulfonylfluoride, EDTA, 1,10-phenanthroline	Homo sapiens
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane	i.e. E-64	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0083	-	benzyloxycarbonyl-Leu-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.013	-	Benzyloxycarbonyl-Leu-Leu-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.03	-	tert-butyloxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide	-	Homo sapiens
0.033	-	Benzyloxycarbonyl-Val-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.044	-	Benzyloxycarbonyl-Val-Val-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.05	-	Succinyl-Leu-Tyr 4-methylcoumarin 7-amide	-	Homo sapiens
0.058	-	Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.16	-	Acetyl-Phe-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.17	-	Benzyloxycarbonyl-Glu-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.24	-	tert-butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.28	-	Pro-Phe-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.34	-	benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.51	-	benzoyl-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
1.1	-	tosyl-Gly-Pro-Arg 4-methylcoumarin 7-amide	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	1 * 37000, procathepsin K, 1 * 27000, mature enzyme, SDS-PAGE	Homo sapiens
35000	-	procathepsin K, mature enzyme produced by incubation at pH 4.0, 24 h, 4°C, gel filtration	Homo sapiens
37000	-	1 * 37000, procathepsin K, 1 * 27000, mature enzyme, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Protein + H2O	Homo sapiens	involved in bone resorption, hydrolysis of proteinaceous bone matrix in the process of remodeling of the human skeleton	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant procathepsin K, expressed in baculovirus infected SF21 cells, processed to cathepsin K	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Acetyl-Phe-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Acetyl-Phe-Arg + 7-amino-4-methylcoumarin	-	?
Benzoyl-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Benzoyl-Arg + 7-amino-4-methylcoumarin	-	?
Benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin	-	?
Benzyloxycarbonyl-Glu-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Benzyloxycarbonyl-Glu-Arg + 7-amino-4-methylcoumarin	-	?
Benzyloxycarbonyl-Leu-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin	-	?
Benzyloxycarbonyl-Leu-Leu-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Benzyloxycarbonyl-Leu-Leu-Arg + 7-amino-4-methylcoumarin	-	?
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin	-	?
Benzyloxycarbonyl-Val-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Benzyloxycarbonyl-Val-Arg + 7-amino-4-methylcoumarin	-	?
Benzyloxycarbonyl-Val-Val-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin	-	?
Collagen + H2O	poor substrate	Homo sapiens	Fragments of collagen	-	?
Fibrinogen + H2O	poor substrate	Homo sapiens	Fragments of fibrinogen	-	?
Osteonectin + H2O	-	Homo sapiens	Fragments of osteonectin	the cleavage products are of MW 34000 and 14000. The 34000 Da protein represents the amino-terminus of osteonectin minus three amino acids Ala-Pro-Gln. Cleavage of the Arg-Val bond in the sequence Gln-Lys-Leu-Arg-Val-Lys-Ole-His of the 14000 Da fragment. The presence of Lsu-Arg at position P2-P1 is favorable	?
Pro-Phe-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Pro-Phe-Arg + 7-amino-4-methylcoumarin	-	?
Protein + H2O	involved in bone resorption, hydrolysis of proteinaceous bone matrix in the process of remodeling of the human skeleton	Homo sapiens	?	-	?
Succinyl-Leu-Tyr 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Succinyl-Leu-Tyr + 7-amino-4-methylcoumarin	-	?
tert-Butyloxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	tert-Butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin	-	?
tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin	-	?
Tosyl-Gly-Pro-Arg 4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 37000, procathepsin K, 1 * 27000, mature enzyme, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Human osteoclast cathepsin K	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.008	-	Succinyl-Leu-Tyr 4-methylcoumarin 7-amide	-	Homo sapiens
0.016	-	Acetyl-Phe-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.021	-	Benzyloxycarbonyl-Val-Val-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.037	-	Benzyloxycarbonyl-Glu-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.078	-	Benzyloxycarbonyl-Leu-Leu-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.083	-	tert-butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.092	-	benzoyl-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.16	-	Pro-Phe-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.39	-	benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
0.52	-	tert-butyloxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide	-	Homo sapiens
2.2	-	tosyl-Gly-Pro-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
2.9	-	Benzyloxycarbonyl-Val-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
3.4	-	benzyloxycarbonyl-Leu-Arg 4-methylcoumarin 7-amide	-	Homo sapiens
3.5	-	Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide	-	Homo sapiens

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Release 2023.1 (January 2023)