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Literature summary for 3.4.22.37 extracted from

  • Veillard, F.; Sztukowska, M.; Mizgalska, D.; Ksiazek, M.; Houston, J.; Potempa, B.; Enghild, J.J.; Thogersen, I.B.; Gomis-Rueth, F.X.; Nguyen, K.A.; Potempa, J.
    Inhibition of gingipains by their profragments as the mechanism protecting Porphyromonas gingivalis against premature activation of secreted proteases (2013), Biochim. Biophys. Acta, 1830, 4218-4228.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.37

Inhibitors

Inhibitors Comment Organism Structure
additional information the prodomain of lysine-specific gingipain shows no inhibitory activity Porphyromonas gingivalis
Phe-Arg-fluoromethylketone
-
 Porphyromonas gingivalis
prodomain of arginine-specific gingipain A
-
 Porphyromonas gingivalis
prodomain of arginine-specific gingipain B
-
 Porphyromonas gingivalis
tosyl-L-lysyl-chloromethylketone
-
 Porphyromonas gingivalis
YPR-chloromethylketone
-
 Porphyromonas gingivalis
Z-FFR-chloromethylketone
-
 Porphyromonas gingivalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Porphyromonas gingivalis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, low molecular weight gingipain B, SDS-PAGE Porphyromonas gingivalis
95000
-
 x * 95000, high molecular weight gingipain A, SDS-PAGE Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-
Porphyromonas gingivalis HG66
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone sulfate precipitation, arginine-Sepharose column chromatography, and Sephadex G-150 gel filtration Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Azocoll + H2O
-
 Porphyromonas gingivalis ?
-
 ?
Azocoll + H2O
-
 Porphyromonas gingivalis HG66 ?
-
 ?
Nalpha-benzoyl-L-arginine 4-nitroanilide + H2O
-
 Porphyromonas gingivalis Nalpha-benzoyl-L-arginine + 4-nitroaniline
-
 ?
Nalpha-benzoyl-L-arginine 4-nitroanilide + H2O
-
 Porphyromonas gingivalis HG66 Nalpha-benzoyl-L-arginine + 4-nitroaniline
-
 ?
Z-Arg-7-amido-4-methylcoumarin + H2O
-
 Porphyromonas gingivalis Z-Arg + 7-amino-4-methylcoumarin
-
 ?
Z-Arg-7-amido-4-methylcoumarin + H2O
-
 Porphyromonas gingivalis HG66 Z-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, low molecular weight gingipain B, SDS-PAGE Porphyromonas gingivalis
? x * 95000, high molecular weight gingipain A, SDS-PAGE Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
Arginine-specific gingipain
-
 Porphyromonas gingivalis
high molecular weight gingipain A
-
 Porphyromonas gingivalis
HRgpA isoform Porphyromonas gingivalis
low molecular weight gingipain B
-
 Porphyromonas gingivalis
RgpB isoform Porphyromonas gingivalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000085
-
 prodomain of arginine-specific gingipain A isoform HRgpA, in 200 mM Tris-HCl, 5 mM CaCl2, 150 mM NaCl, and 0.02% (w/v) NaN3, pH 7.6, supplemented with 10 mM L-cysteine, at 37°C Porphyromonas gingivalis
0.0000021
-
 prodomain of arginine-specific gingipain A isoform RgpB, in 200 mM Tris-HCl, 5 mM CaCl2, 150 mM NaCl, and 0.02% (w/v) NaN3, pH 7.6, supplemented with 10 mM L-cysteine, at 37°C Porphyromonas gingivalis
0.0000053
-
 prodomain of arginine-specific gingipain B isoform HRgpA, in 200 mM Tris-HCl, 5 mM CaCl2, 150 mM NaCl, and 0.02% (w/v) NaN3, pH 7.6, supplemented with 10 mM L-cysteine, at 37°C Porphyromonas gingivalis
0.0000062
-
 prodomain of arginine-specific gingipain B isoform RgpB, in 200 mM Tris-HCl, 5 mM CaCl2, 150 mM NaCl, and 0.02% (w/v) NaN3, pH 7.6, supplemented with 10 mM L-cysteine, at 37°C Porphyromonas gingivalis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000071
-
 isoform RgpB, with azocoll as substrate, in 200 mM Tris-HCl, 5 mM CaCl2, 150 mM NaCl, and 0.02% (w/v) NaN3, pH 7.6, supplemented with 10 mM L-cysteine, at 37°C Porphyromonas gingivalis prodomain of arginine-specific gingipain B
0.0000072
-
 isoform RgpB, with H-Arg-7-amido-4-methylcoumarin as substrate, in 200 mM Tris-HCl, 5 mM CaCl2, 150 mM NaCl, and 0.02% (w/v) NaN3, pH 7.6, supplemented with 10 mM L-cysteine, at 37°C Porphyromonas gingivalis prodomain of arginine-specific gingipain B
0.0000237
-
 isoform RgpB, with Nalpha-benzoyl-L-arginine 4-nitroanilide as substrate, in 200 mM Tris-HCl, 5 mM CaCl2, 150 mM NaCl, and 0.02% (w/v) NaN3, pH 7.6, supplemented with 10 mM L-cysteine, at 37°C Porphyromonas gingivalis prodomain of arginine-specific gingipain B