Literature summary for 3.4.22.37 extracted from

Banbula, A.; Potempa, J.; Travis, J.; Bode, W.; Medrano, F.J.
Crystallization and preliminary X-ray diffraction analysis of gingipain R2 from Porphyromonas gingivalis in complex with H-D-Phe-Phe-Arg-chloromethylketone (1998), Protein Sci., 7, 1259-1261.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme gingipain R2 in complex with fast acting, irreversible inhibitor H-D-Phe-Phe-Arg-chloromethylketone, activated by incubation for 30 min in 0.1 M HEPES, pH 8.0, 10 mM L-cysteine, 2.5 mM CaCl2, at 37°C, alkylation of the active site cysteine by addition of inhibitor, crystallization by sitting-drop vapour diffusion method at 20°C, different protein-inhibitor complex solution systems using PEG 8000 as precipitant for 10 mg/ml protein, X-ray diffraction structure determination at 2.9 A resolution and analysis	Porphyromonas gingivalis

Crystallization (Comment)

Organism

purified enzyme gingipain R2 in complex with fast acting, irreversible inhibitor H-D-Phe-Phe-Arg-chloromethylketone, activated by incubation for 30 min in 0.1 M HEPES, pH 8.0, 10 mM L-cysteine, 2.5 mM CaCl2, at 37°C, alkylation of the active site cysteine by addition of inhibitor, crystallization by sitting-drop vapour diffusion method at 20°C, different protein-inhibitor complex solution systems using PEG 8000 as precipitant for 10 mg/ml protein, X-ray diffraction structure determination at 2.9 A resolution and analysis

Porphyromonas gingivalis

Inhibitors

Inhibitors	Comment	Organism	Structure
D-Phe-Phe-Arg-chloromethylketone	fast acting, irreversible alkylating inhibitor	Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protein + H2O	Porphyromonas gingivalis	-	peptides	-	?

Organism

Organism	UniProt	Comment	Textmining
Porphyromonas gingivalis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Porphyromonas gingivalis

Reaction

Reaction	Comment	Organism	Reaction ID
hydrolysis of proteins and small molecule substrates, with a preference for Arg in P1	active site structure	Porphyromonas gingivalis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protein + H2O	-	Porphyromonas gingivalis	peptides	-	?

Synonyms

Synonyms	Comment	Organism
gingipain R2	-	Porphyromonas gingivalis
RGP-2	-	Porphyromonas gingivalis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Porphyromonas gingivalis	gingipain R2 isozyme II, isoelectric focusing	-	5.1