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Literature summary for 3.4.22.37 extracted from

  • Houle, M.A.; Grenier, D.; Plamodon, P.; Nakayama, K.
    The collagenase activity of Porphyromonas gingivalis is due to Arg-gingipain (2003), FEMS Microbiol. Lett., 221, 181-185.
    View publication on PubMed
Search for more literature for 3.4.22.37

Protein Variants

Protein Variants Comment Organism
additional information 4 naturally occuring enzyme deficient mutants: rgpA-deficient mutant shows 77% activity compared to the wild-type activity with type I collagen, while a rgpB-deficent mutant shows 82% activity, and a double-deficient mutant strain shows only 6% activity, another mutant lacking the to arginine-specific isozyme and the lysine-specific gingipain KGP shows 5% remaining activity Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
Chlorhexidine strong inhibition Porphyromonas gingivalis
doxycycline strong inhibition Porphyromonas gingivalis
leupeptin strong inhibition Porphyromonas gingivalis
additional information no or nearly no inhibition by cathepsin B inhibior II, metronidazole, peicillin G, and erythromycin Porphyromonas gingivalis
tetracycline
-
 Porphyromonas gingivalis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular attached to the cell surface Porphyromonas gingivalis
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen type I + H2O Porphyromonas gingivalis not the purified isoforms, enzyme probably needs to be attached to the cell surface ?
-
 ?
Collagen type I + H2O Porphyromonas gingivalis ATCC 33277 not the purified isoforms, enzyme probably needs to be attached to the cell surface ?
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
 strain ATCC33277, wild-type and 4 enzyme-deficient mutants, isozymes gingipains A and B, encoded by the genes rgpA and rgpB
-
Porphyromonas gingivalis ATCC 33277
-
 strain ATCC33277, wild-type and 4 enzyme-deficient mutants, isozymes gingipains A and B, encoded by the genes rgpA and rgpB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen type I + H2O not the purified isoforms, enzyme probably needs to be attached to the cell surface Porphyromonas gingivalis ?
-
 ?
Collagen type I + H2O not the purified isoforms, enzyme probably needs to be attached to the cell surface Porphyromonas gingivalis ATCC 33277 ?
-
 ?
Gelatin + H2O purified isozymes Porphyromonas gingivalis ?
-
 ?
Gelatin + H2O purified isozymes Porphyromonas gingivalis ATCC 33277 ?
-
 ?

Synonyms

Synonyms Comment Organism
Arg-gingipain
-
 Porphyromonas gingivalis
Arg-gingivain-specific proteinase
-
 Porphyromonas gingivalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37 with denatured substrate collagen type I, native collagen type I is only degraded at 37°C Porphyromonas gingivalis