Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.37 extracted from

  • Fujimura, S.; Hirai, K.; Shibata, Y.; Nakayama, K.; Nakamura, T.
    Comparative properties of envelope-associated arginine-gingipains and lysine-gingipain of Porphyromonas gingivalis (1998), FEMS Microbiol. Lett., 163, 173-179.
    View publication on PubMed
Search for more literature for 3.4.22.37

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Porphyromonas gingivalis
cysteine
-
 Porphyromonas gingivalis
dithiothreitol
-
 Porphyromonas gingivalis
glycyl-glycine activates only RGP-B Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
antipain both enzyme forms Porphyromonas gingivalis
EDTA both enzyme forms Porphyromonas gingivalis
L-trans-epoxy-succinylleucylamido-(4-guanidino)butane both enzyme forms Porphyromonas gingivalis
leupeptin both enzyme forms Porphyromonas gingivalis
additional information no inhibition by aprotinin, epsilon-aminocaproic acid and diisopropylfluorophosphate Porphyromonas gingivalis
N-ethylmaleimide both enzyme forms Porphyromonas gingivalis
p-hydroxymercuribenzoate complete inhibition at 0.2 mM, both enzyme forms Porphyromonas gingivalis
tosyl-L-lysine chloromethyl ketone i.e. TLCK, both enzyme forms Porphyromonas gingivalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell envelope
-
 Porphyromonas gingivalis 30313
-
extracellular
-
 Porphyromonas gingivalis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme forms are not affected by Mg2+ and Ca2+ Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, both enzyme forms, SDS-PAGE Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Porphyromonas gingivalis
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
 strain ATCC 33277, 2 arginine-specific gingipains RGP-A and RGP-B
-

Purification (Commentary)

Purification (Comment) Organism
RGP-A and RGP-B, from envelope material, solubilization by detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, i.e. CHAPS Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-L-arginine-4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-L-arginine + 4-nitroaniline
-
 ?
benzoyl-Phe-Val-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-Phe-Val-Arg + 4-nitroaniline
-
 ?
benzoyl-Pro-Phe-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-Pro-Phe-Arg + 4-nitroaniline
-
 ?
benzoyl-Val-Gly-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-Val-Gly-Arg + 4-nitroaniline
-
 ?
butoxy-carbonyl-O-benzyl-Ser-Gly-Arg-4-nitroanilide + H2O best sythetic substrate Porphyromonas gingivalis butoxy-carbonyl-O-benzyl-Ser-Gly-Arg + 4-nitroaniline
-
 ?
butoxy-carbonyl-Val-Leu-Gly-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis butoxy-carbonyl-Val-Leu-Gly-Arg + 4-nitroaniline
-
 ?
haemoglobin + H2O complete digestion, enzyme form RGP-B shows high activity, not RGP-A, human substrate Porphyromonas gingivalis ?
-
 ?
additional information no activity of both enzyme forms with toluenesulfonyl-glycyl-L-prolyl-L-lysine-4-nitroanilide, Val-Leu-Lys-4-nitroanilide, benzoyl-Lys-4-nitroanilide, succinyl-Ala-Ala-Pro-Phe-4-nitroanilide, glutaryl-Phe-4-nitroanilide, benzoyl-Tyr-4-nitroanilide, and Lys-4-nitroanilide, activity with chromogenic synthetic substrates is limited to those with arginine in the P1-position Porphyromonas gingivalis ?
-
 ?
protein + H2O
-
 Porphyromonas gingivalis peptides
-
 ?
toluenesulfonyl-glycyl-L-prolyl-L-arginine-4-nitroanilide + H2O
-
 Porphyromonas gingivalis toluenesulfonyl-glycyl-L-prolyl-L-arginine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, both enzyme forms, SDS-PAGE Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
Arg-gingipain
-
 Porphyromonas gingivalis
arginine-gingipain
-
 Porphyromonas gingivalis
Arginine-specific gingipain
-
 Porphyromonas gingivalis
RGP
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at, substrate haemoglobin Porphyromonas gingivalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 50 mM Tris,-HCl, pH 7.8, 10 min, remaining activity for RGP-A is 82% and for RGP-B 52% of maximal activity, after 30 min remaining activity for RGP-A is 58% and for RGP-B 26% of maximal activity Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 both enzyme forms Porphyromonas gingivalis

pH Range

pH Minimum pH Maximum Comment Organism
8.5
-
 rapid decrease of activity above pH 8.5 Porphyromonas gingivalis

pI Value

Organism Comment pI Value Maximum pI Value
Porphyromonas gingivalis RGP-B, isoelectric focusing
-
 3.8
Porphyromonas gingivalis RGP-A, isoelectric focusing 4.8 4.7