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Literature summary for 3.4.22.34 extracted from
- Legumain forms from plants and animals differ in their specificity (2001), Biol. Chem., 382, 953-959.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ovocystatin | weak inhibition, from egg-white | Phaseolus vulgaris | |
| ovocystatin | from egg-white | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | Phaseolus vulgaris | - |
peptides | - |
? |  |
| protein + H2O | Sus scrofa | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
purfied enzyme | - |
| Sus scrofa | - |
purified enzyme | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
purified enzyme | Phaseolus vulgaris |
| 7 | - |
purified enzyme | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide + H2O | - |
Phaseolus vulgaris | acetyl-Tyr-Val-Ala-Asp + 7-amino-4-methylcoumarin | - |
? | |
| acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide + H2O | - |
Sus scrofa | acetyl-Tyr-Val-Ala-Asp + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Sus scrofa | benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O | best synthetic substrate | Phaseolus vulgaris | benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Phaseolus vulgaris | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | best synthetic substrate | Sus scrofa | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Gly-Ala-Asn-4-methylcoumarin-7-amide + H2O | low activity | Phaseolus vulgaris | benzyloxycarbonyl-Gly-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Gly-Ala-Asn-4-methylcoumarin-7-amide + H2O | low activity | Sus scrofa | benzyloxycarbonyl-Gly-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Leu-Ala-Asn-4-methylcoumarin-7-amide + H2O | low activity | Phaseolus vulgaris | benzyloxycarbonyl-Leu-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Leu-Ala-Asn-4-methylcoumarin-7-amide + H2O | low activity | Sus scrofa | benzyloxycarbonyl-Leu-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Phe-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Phaseolus vulgaris | benzyloxycarbonyl-Phe-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Phe-Ala-Asn-4-methylcoumarin-7-amide + H2O | low activity | Sus scrofa | benzyloxycarbonyl-Phe-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Pro-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Sus scrofa | benzyloxycarbonyl-Pro-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Pro-Ala-Asn-4-methylcoumarin-7-amide + H2O | low activity | Phaseolus vulgaris | benzyloxycarbonyl-Pro-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Phaseolus vulgaris | benzyloxycarbonyl-Tyr-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O | low activity | Sus scrofa | benzyloxycarbonyl-Tyr-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Phaseolus vulgaris | benzyloxycarbonyl-Val-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Sus scrofa | benzyloxycarbonyl-Val-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| additional information | substrate specificity for P3 position residues | Phaseolus vulgaris | ? | - |
? | |
| additional information | substrate specificity for P3 position residues | Sus scrofa | ? | - |
? | |
| neurotensin + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
| neurotensin + H2O | - |
Sus scrofa | ? | - |
? | |
| protein + H2O | - |
Phaseolus vulgaris | peptides | - |
? | |
| protein + H2O | - |
Sus scrofa | peptides | - |
? | |
| tetanus toxoid C fragment + H2O | cleavage sites are at Asn26, Asn97, and Asn372, but not Asn337 | Phaseolus vulgaris | 3 fragments | - |
? | |
| tetanus toxoid C fragment + H2O | cleavage sites are at Asn26, Asn337, and Asn372 | Sus scrofa | tetanus toxoid fragments | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Asparaginyl endopeptidase | - |
Phaseolus vulgaris |
| Asparaginyl endopeptidase | - |
Sus scrofa |
| Proteinase B | - |
Phaseolus vulgaris |
| Proteinase B | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phaseolus vulgaris |
| 30 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
with substrate acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide | Phaseolus vulgaris |
| 5 | - |
with substrate acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide | Sus scrofa |
| 5.4 | - |
with substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide | Phaseolus vulgaris |
| 5.8 | - |
with substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide | Sus scrofa |
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Release 2023.1 (January 2023)
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