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Literature summary for 3.4.22.34 extracted from

  • Dando, P.M.; Fortunato, M.; Smith, L.; Knight, C.G.; McKendrick, J.E.; Barrett, A.J.
    Pig kidney legumain: an asparaginyl endopeptidase with restricted (1999), Biochem. J., 339, 743-749.
No PubMed abstract available
Search for more literature for 3.4.22.34

Inhibitors

Inhibitors Comment Organism Structure
additional information glycosylation of asparaginyl residues totally prevents cleavage by the enzyme, no binding to human alpha2-macroglobulin Sus scrofa
rat alpha1-macroglobulin
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Sus scrofa 5764
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Sus scrofa
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 purified enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney cortex Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7
-
 purified enzyme Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AGTHNGQIGA + H2O the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived, low activity Sus scrofa AGTHN + GQIGA
-
 ?
AHIDNEEDIA + H2O low activity, the peptide sequence is also cleaved in tetanus toxoid C fragment of which it is derived Sus scrofa AHIDN + EEDIA
-
 ?
AHIDNESDIA + H2O low activity Sus scrofa AHIDN + ESDIA
-
 ?
ALKGNNLIWA + H2O the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived Sus scrofa ALKGN + NLIWA
-
 ?
AQLKNITDYA + H2O the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived Sus scrofa AQLKN + ITDYA
-
 ?
AREDNNITLA + H2O low activity, the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived Sus scrofa AREDN + NITLA
-
 ?
ARLYNGLKFA + H2O the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived Sus scrofa ARLYN + GLKFA
-
 ?
ASGFNSSVIA + H2O the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived Sus scrofa ASGFN + SSVIA
-
 ?
ATITNDRLSA + H2O the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived Sus scrofa ATITN + DLRSA
-
 ?
AYGTNEYSIA + H2O the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived Sus scrofa AYGTN + EYSIA
-
 ?
benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Gly-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Gly-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Leu-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Phe-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Pro-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Pro-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Tyr-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Val-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
bovine serum albumin + H2O SDS-denatured substrate, cleavage site are at positions 324 and 404 Sus scrofa 3 fragments
-
 ?
casein 1 + H2O cleavage site is at position 95 Sus scrofa 2 fragments
-
 ?
concanavalin A A-chain + H2O cleavage site are at positions 159 and 163 Sus scrofa 3 fragments
-
 ?
lysozyme C + H2O cleavage site are at positions 62 and 64 Sus scrofa 3 fragments
-
 ?
additional information substrate specificity, overview, activity with oligopeptides derived from several protein substrates, overview, no activity with peptides AWYFNHLKDA, and ANDPNRDILA, no activity with substrate analogues containing mono-or di-N-methylasparagines, L-2-amino-3-ureidopropionic acid or citrulline in the P1 position Sus scrofa ?
-
 ?
protein + H2O
-
 Sus scrofa peptides
-
 ?
protein + H2O strong specificity for asparaginyl residues located at the protein surface Sus scrofa peptides
-
 ?
rat alpha1-macroglobulin + H2O cleavage site are at positions 721 and 899 Sus scrofa 3 fragments
-
 ?
tetanus toxoid C fragment + H2O cleavage site are at positions 26, 337, and 372 Sus scrofa ?
-
 ?
transferrin + H2O cleavage site are at positions 95, 529, 574, and 603 Sus scrofa 5 fragments
-
 ?

Synonyms

Synonyms Comment Organism
Asparaginyl endopeptidase
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 assay at Sus scrofa