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Literature summary for 3.4.22.34 extracted from
- Legumain: asparaginyl endopeptidase (1994), Methods Enzymol., 244, 604-615.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Canavalia ensiformis |
| Brij 35, 0.005-0.05%, stabilizes | Canavalia ensiformis |
| DTT stabilizes | Canavalia ensiformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cystatin | EW | Canavalia ensiformis | |
| diisopropyl fluorophosphate | - |
Canavalia ensiformis |  |
| High molecular weight kininogen | - |
Canavalia ensiformis | |
| leupeptin | - |
Canavalia ensiformis | |
| NEM | - |
Canavalia ensiformis | |
| p-chloromercuribenzene sulfonic acid | - |
Canavalia ensiformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
Dinitrophenyl-Pro-Glu-Ala-Asn-NH2 | pH 5.0 | Canavalia ensiformis | |
| 0.033 | - |
Dinitrophenyl-Pro-Glu-Ala-Asn-NH2 | pH 5.9 | Canavalia ensiformis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Canavalia ensiformis | thought to be involved in the hydrolysis of stored seed proteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canavalia ensiformis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Canavalia ensiformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Canavalia ensiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Dinitrophenyl-Pro-Glu-Ala-Asn-NH2 + H2O | - |
Canavalia ensiformis | Dinitrophenyl-Pro-Glu-Ala-Asn + NH4OH | - |
? | |
| additional information | specificity overview with various peptide substrates, strict specificity towards asparagine bonds | Canavalia ensiformis | ? | - |
? | |
| additional information | thought to be involved in the hydrolysis of stored seed proteins | Canavalia ensiformis | ? | - |
? | |
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Release 2023.1 (January 2023)
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