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Literature summary for 3.4.22.34 extracted from
- Purification and characterization of vicilin peptidohydrolase, the major endopeptidase in the cotyledons of mung-bean seedlings (1977), Eur. J. Biochem., 77, 223-233.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 10 mM, 300% activation | Vigna radiata |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not: EDTA; N-tosyl-L-lysine chloromethyl ketone; phenylmethylsulfonyl fluoride | Vigna radiata | |
| NEM | - |
Vigna radiata | |
| PCMB | - |
Vigna radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
1 * 22500, Vigna radiata, SDS-PAGE | Vigna radiata |
| 23500 | - |
Vigna radiata, gel filtration | Vigna radiata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Vicilin + H2O | Vigna radiata | degradation of the principal reserve protein present in Vigna radiata seeds | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Vigna radiata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Vigna radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonyl-Ala 4-nitrophenyl ester + H2O | - |
Vigna radiata | Benzyloxycarbonyl-Ala + 4-nitrophenol | - |
? | |
| Benzyloxycarbonyl-Asn nitrophenyl ester + H2O | - |
Vigna radiata | Benzyloxycarbonyl-Asn + 4-nitrophenol | - |
? | |
| Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O | - |
Vigna radiata | Benzyloxycarbonyl-Gly + 4-nitrophenol | - |
? | |
| benzyloxycarbonyl-Phe 4-nitrophenyl ester + H2O | - |
Vigna radiata | benzyloxycarbonyl-Phe + 4-nitrophenol | - |
? | |
| Benzyloxycarbonyl-Tyr 4-nitrophenyl ester + H2O | - |
Vigna radiata | Benzyloxycarbonyl-Tyr + 4-nitrophenol | - |
? | |
| tert-butoxycarbonyl-Asn nitrophenyl ester + H2O | - |
Vigna radiata | tert-butoxycarbonyl-Asn + 4-nitrophenol | - |
? | |
| Tert-Butoxycarbonyl-Gln 4-nitrophenyl ester + H2O | - |
Vigna radiata | Tert-Butoxycarbonyl-Gln + 4-nitrophenol | - |
? | |
| Vicilin + H2O | - |
Vigna radiata | Hydrolyzed vicilin | - |
? | |
| Vicilin + H2O | degradation of the principal reserve protein present in Vigna radiata seeds | Vigna radiata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 22500, Vigna radiata, SDS-PAGE | Vigna radiata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Vigna radiata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
10 min, stable up to | Vigna radiata |
| 50 | - |
10 min, complete inactivation | Vigna radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | - |
vicilin | Vigna radiata |
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